88 MICROSCOPICAL TECHNIQUE. 



these new nuclei then becomes united to the pedicel as at the 

 mouth of a funnel, and a slender thread of chromatin presently 

 extends from the point of union to the daughter cell. There it 

 becomes swollen, and gradually attracts to itself all the chromatic 

 granules. During the passage through the pedicel, the nucleus 

 shows no trace of nuclear membrane, but subsequently this 

 appears as usual. The nucleus remaining in the mother cell is 

 carried to another point where a fresh bud has formed. When 

 growth is rapid, several buds may be observed in a cell at the 

 same time, but they are of different ages, having been formed 

 successivel} in the manner described, each new budding corres- 

 ponding to a fresh division of the nucleus. — Pharm. Journ. 



Detection of Cholera Bacillus."^— Koch's former method of 

 examination, though certain in its results, was somewhat tedious, 

 and a more speedy method has been tried at the Institute for 

 Infectious Diseases, Berlin. A little mucus from the liquid part 

 of the object to be examined is fixed on a cover-glass and stained 

 with Ziehl's fuchsine solution. Cholera bacteria, in such a prepa- 

 ration, would either occur alone or mixed with ordinary intestinal 

 bacteria, especially B. coli. They are said to lie as a rule where 

 the mucus is drawn into threads, and in the characteristically 

 formed groups the individual bacilli lie in one direction. But even 

 when the bacteria are scattered, and perhaps mixed with the B. 

 coli, it is stated that Asiatic cholera may be diagnosed with cer- 

 tainty. Only when the specimen is a very mixed one is there any 

 uncertainty in the matter. As regards the method of culture 

 adopted, a peptone process is now employed by Koch in which the 

 lumps of mucus are placed in a one per cent, sterilised peptone 

 solution in a reagent glass, and kept at a temperature of 37° C. 

 When the preparation begins to grow turbid, the bacteria may be 

 discovered on the surface (in about six hours) if they are numer- 

 ous in the test specimen, but when only few they are discovered 

 later mixed with other bacteria. These peptone cultivations are 

 said to have given positive results when gelatin plates failed, but 

 the most satisfactory procedure is to combine the two processes. — 

 Pharmaceutical Journal. 



* Zeit. f. Hygiene, through Med. Press, Vol. CVI., p. 643. 



