APPENDIX I. 



Part I. of this work was published in 1896, Part II. in 1899, 

 Part III. in 1904, Part IV. in 1909, and Part V. bears the 

 date 1922. 



During the years that have elapsed since the issue of the 

 earlier parts, increased knowledge has necesitated various correc- 

 tions, emendations, and additions, which are presented in the 

 following notes : — 



Preparation for Microscopical Study (p. 10). 



During the course of years I have modified my earlier methods consider- 

 ably, partly as a result of my own experience, and partly from a study of 

 the methods of other microscopists. The process that I now employ is as 

 follows : — 



(i) Boil the specimens in strong liquor potassic for from three minutes 

 upwards (according to the size of the subject). 



N.B. — It is better to under-boil the specimens than to over-boil them. The 

 former fault can be rectified by further treatment, but, if the maceration has 

 proceeded too far, the objects will not take the stain properly. 



(2) Remove the objects to distilled water, and put them aside for twenty- 

 four hours. 



(3) Make a small opening on the under surface of the body, and squeeze or 

 wash out the contents. 



(4) Transfer to glacial acetic acid for two or three minutes. 



(5) Mount temporarily on a glass slip, add two drops of carbol-fuchsin ; 

 add one drop of dilute glycerine (to prevent evaporation of the stain) ; warm 

 gently, and put aside for twenty-four hours. 



(6) Wash in 70 per cent, alcohol (to remove superfluous stain), transfer to 

 absolute alcohol, if too deeply stained immerse momentarily in phenol-chloral ; 

 transfer to phenol-xylol for a few minutes ; mount finally in xylol-balsam. 



Synopsis of Sub-families (pp. 16, 17). 



The sub-family Hemicoccimc has been abandoned, having been founded 

 upon a misinterprepretation of the structure of the insects referred thereto. 



Idiococcince also has been abandoned, as consisting of species referable to 

 other sub-families. 



The name Coccincc is no longer applicable to the Cochineal insects, 

 which are now referred to the sub-family Dactylopiimc. 



The Eriococcine and Asterolecaniine groups may be conveniently separated 

 from the Daciylopiina; and given rank as sub-families. 



A sub-family Margarodincc should be interpolated between the Ortheziime 

 and the MotiopJilebincc. 



Aspidiotus osbeckise (p. 47). 



This species is referable to oricntalis of Newstead. 



