50 SMITHSOXIAX MISCELLANEOUS COLLECTIONS VOL. 52 



Mr. Allen H. Jennings, of the department, was detailed to be 

 with me as much as possible in order to learn our methods in the 

 routine work of collecting, breeding, and taking care of the mosqui- 

 toes ; his frequent companionship in the field and in the laboratory 

 was very pleasant and facilitated my work in many ways. He was 

 good enough to take charge of my living larvse on two occasions of 

 more prolonged absence. 



The several local sanitary inspectors along the Zone line gave me 

 much assistance by collecting material and giving me facilities for 

 work when I visited their stations. 



Through the foresight of Doctor Canfiekl a system was inau- 

 gurated whereby each sanitary inspector sent me weekly a bottle of 

 mosquito larv?e, and though this material could not be expected to be 

 of especial value, it furnished additional localities for the common 

 species and occasionally yielded rarer ones. I must especially men- 

 tion Mr. C. H. Bath, sanitary inspector at Las Cascadas, whose care- 

 ful and regular sendings yielded several interesting larvae. 



The number of species of mosquitoes secured was 83, of which 30 

 species were new to science. Most of the species were bred from 

 the larvae. Besides these I have included in the following list, in order 

 to make it as complete as possible, 7 additional species, previously 

 received by the U. S. Department of Agriculture from Panama 

 through other collectors, bringing the total number of species at 

 present known from Panama up to 90. The collection was de- 

 termined by Dr. H. G. Dyar and Mr. F. Knab.^ All the types of 

 new species are deposited in the U. S. National ^luseum, as well as 

 all the other material, with the exception of a duplicate set presented 

 to the Isthmian Canal Commission. 



Large as this number of species is — the largest number recorded 

 from any one limited locality — there is yet much work to be done 

 before the entire mosquito fauna of the Zone is known. 



It was impossible to work up thoroughly so large an area within 

 three months, and only the immediate region around Tabernilla was 

 at all adequately investigated. Even here additional species will 

 undoubtedlx- be found, because the fauna changes considerably with 

 the season, and some species may not have been active at all during 

 the period of my visit, though this was intentionally arranged so as 

 to cover both the end of the dry season and the early part of the 

 rainy season. The appearance of different species of tropical mos- 

 quitoes at different seasons is a well-marked phenomenon and was 

 repeatedly observed even during my short stay. 



^The new species were described in Journ. N. Y. Ent. Soc, vol. xv, 1907, pp. 

 197-214. 



