liTf) Journal of Applied Microscopy 



occupies the central portion of the root, of which, however, the innermost part is 

 broken down into a cavity ; thus the principal features of the primary root are 

 almost totally obliterated. Oil-ducts are quite numerous in the mature root ; 

 they are located in the same radii as the mestome bundles and occur in four or 

 five concentric bands. The innermost oil-ducts are to be seen in the leptome 

 itself, the others some distance apart, the outermost being very near the peri- 

 phery, though not in contact with the cork. It appears as if the ducts of the 

 outermost two bands are mostly pentagonal in transverse sections ; while those 

 of the inner are rhombic and somewhat narrower in circumference." 



There are thirty-eight oil-ducts in each mericarp in each fruit, twelve on the 

 commisural side, one outside each of the five mestome bundles, and from five to 

 six in the intervals between these. The mericarps are not glabrous, but hairy, 

 consisting of short unicellular pointed hairs which cover the entire dorsal face. 

 Agri. College, Ames, la. L. H. Pammel. 



CYTOLOGY, EMBRYOLOGY, 



AND 



MICROSCOPICAL METHODS. 



Agnes M. Claypole. 



Separates of papers and books on animal biology should be sent for review to 



Agnes M. Claypole, Sage College, 

 Ithaca, N. Y. 



CURRENT LITERATURE. 



Prenant, A. Cellules Tracheales des Oestres. Material used for this investigation was 

 Archiv. D'Anat. Microscop. 3: 201-3-16 ^ , ... . ^, ^ 1 r ^i 



(Planch. 15-16), 1900. taken, livmg, from the stomach of the 



horse, and consisted in larvae of the 



bot-fly {Gastrophilus eqi/i). Examining these larvae an area of red coloration 



is always seen at the posterior extremity of the animal. On dissection two bodies 



are found, one on each side of the digestive tube, which are white and opaque in 



their anterior three-quarters, and lobulated in structure. In the posterior part 



they are more granular, and of a reddish color, varying to a purple-red. This 



part of the organ is provisionally called the " organe rouge." This organ is 



found to be composed of a number of large oval cells, surrounded thickly by 



tracheae, which branch and finally enter into the interior of the cell. Such cells 



are called " tracheal cells " to distinguish them from the fat cells of the anterior 



part of the organ. Besides using fresh material, organs were fixed in Flemming's 



stronger solution; Mann's fluid (picric acid, sat. sol., 10 pts.; sublimate sat. sol., 



10 pts.; formol, 5 pts.) ; formo-picric sol. of Bouin (sat. sol. picric acid, 75 vols.; 



formol "25 ; glacial acetic acid, 5 pts.) ; platinum mixture of Bouin (platinic 



chloride of 1 per cent, sol., 10 pts. ; sat. sol. picric acid, 20 pts. ; formol, 10 pts.; or 



platinic chloride of 1 per cent, sol., 20 pts.; sat. sol of sublimate, 20 pts.; formol, 



10 pts.; acetic or formic acid, 2 to 5 pts.) ; Weigert's neuroglia fluid, consisting of 



T) per cent. sol. of acetate of copper, 5 per cent, acetic acid, chrome alum 5 per 



cent., and 10 per cent, formol; saline saturated sublimate solution ; Golgi's fluid. 



