1180 Journal of Applied Microscopy 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



Joseph H. Pratt. 



Harvard University Medical School, Boston, Mass.; to whom all books and 

 papers on these subjects should be sent for review. 



Bielschowsky and Pllen. Zur Technik der Ehrlich and Lazarus introduced the 

 Nervenzellenfarbung. Neurologisches Cent- , i • i , r • • . 



ralblatt, 19: 1441, 1900. "se of cresyl-violet for staining the 



basophilic granules of mast-cells. Lit- 

 ten has used the same anilin dye for coloring the basophilic granules which are 

 found in the red blood corpuscles in cases of anaemia. The writers of this article 

 have found cresyl-violet a good stain for the chromophilic substance of the nerve- 

 cells. They used chiefly the preparation which bears the trade name " cresyl- 

 violet R. R." 



In composition this new stain is probably related to methylen blue ; in 

 staining properties it resembles thionin or toluidin blue, but is superior to them, 

 chiefly because the preparations are more permanent, and as dilute solutions 

 are employed it is more economical. Another advantage, which the writers 

 claim, is that the sections are never lost in the dilute transparent solution of 

 cresyl-violet. 



The best results were obtained with the following method : 



1. Harden in alcohol or formalin. 



2. Imbed in celloidin. 



3. Stain in a thin aqueous solution of cresyl-violet for i!4 hours. It is suffi- 

 cient to add six to ten drops of a concentrated aqueous solution to 50 c. c. of 

 water. 



4. Wash quickly in water. 



5. Dehydrate in a series of alcohols of increasing strength. The alcohol, by 

 removing excess of color from the diffusely stained sections, differentiates the 

 gray and white matter of the central nervous system. 



6. Clear in oil of cajeput. 



7. Xylol. 



8. Mount in Canada balsam. 



Equally good results are obtained after imbedding in paraffin. When a quick 

 method is desirable, a concentrated solution of the stain may be used. 



Cresyl-violet gives a metachromatic effect with amyloid, coloring amyloid 

 substance bright blue, the remainder of the section violet. j. h. p. 



Krompecher. Glandhke Carcinoma of Epi- Krompecher describes a peculiar type 

 dermic Origin. Ziegler's Beitrage, 28: i, , , , ,, , . , 1 • 1 i 



j„QQ 00 o Qf tumor of the skin, to which he gives 



the name " carcinoma epithelialeade- 



noides." He believes that the gross and histological appearances are sufficiently 



characteristic to establish it as a distinct group. 



Braun, in 1892, studied this class of tumors, and regarded them as endothel- 



iomata. Krompecher asserts that the diagnosis of endothelioma can be made 



only when the origin of the tumor-masses from the endothelium of the larger 



