and Laboratory Methods. l^'-T 



Willebrand, E. H. Stain for Simultaneous Eosin, 0.5 per cent, alcoholic, 25 C.c. 

 Staining of Blood Smears with Eosin and nr ^i i -r>i i .^k 



Methylen Blue. Deut. Med. Wochens. Methylen Blue, COn. aq. SOl, 25 C.C. 



Jan. 24 and 31, 1901. Acetic acid, 1 per cent., 10-15 drops 



The erythrocytes are stained red, nuclei blue, neuthrophile granules violet, 

 the eosinophile granules red, and those of the mast cells an intense blue. 



c. w. J. 



Lewinson. Method of Staining Fat. Vratch, 

 21, No. 39. 



1. Fix in Miiller's fluid for two to three weeks. 



2. Dehydrate in successive changes of alcohol, commencing with 70 per 



cent. 



3. Imbed in celloidin. 



4. Stain sections of 10 to 15 yu for twelve hours in following solution : 



Haematoxylin . - . . 2 grams 



Alcohol, absolute - sufficient to dissolve haematoxylin 

 Acetic acid, 2 percent, solution - 100 c. c. 



5. Wash in water. 



6. Transfer to 1 per cent, solution permaganate of potash and leave 10 to 



15 minutes. 



7. Wash in water. 



8. Oxalic acid, 2 per cent, solution for five minutes. 



If sections remain yellow or brownish black, carry through the permanganate 

 of potash and oxalic acid solutions again. If no fat is present the sections are 

 colorless. If sections contain fat they are slightly ashy or gray-violet in color. 

 Under the microscope the fat appears gray-violet, while all other structures are 

 unstained. 



The following counterstain may be used : 



1. After removal from oxalic acid solution, wash in water and stain for 24 



hours in an ammonical solution of borax-carmin. 



2. Acid alcohol 1 per cent, for 2 minutes. 



3. Saturated alcoholic solution of picric acid, 1 minute. 



4. Clear in alcohol, xylol, or oil of origanum. 



5. Mount in Canada balsam. 



Nuclei are stained red, protoplasm yellow, and the fat dark, almost black, 



c. w. J. 



Kockel. New Stain for Fibrin. Verhandl. d. 

 Deutsch. Path. Gesell. 11 : 320. 



1. Stain with Weigert's haematoxylin. 



2. Counterstain in Weigert's borax-potassium-ferricyanide solution, diluted 



with three times its volume of water. 

 Fibrin stains dark blue, background light gray or bluish. 

 It is recommended that tissues be hardened in alcohol, sublimate or formalin 

 before being stained by this method, c. w. j. 



