and Laboratory Methods. l'-^61 



difficulty. With glycogen and the fats, however, special methods were necessary. 

 Glycogen was mechanically united to albumin by dissolving large quantities in 

 egg-albumin and coagulating with heat. The mixture was then dried, powdered 

 and fed, the results showing that glycogen had been injested with the albumin. 

 In the case of the fats an emulsion of fish oil in albumin was treated in the same 

 way, and, after feeding the dried albumin, oil globules could be seen within the 

 cytoplasm. 



The results obtained concern almost entirely the refractive bodies which are 

 present in great abundance within the cytoplasm, and are easily and constantly 

 affected by certain kinds of foods. The principal results are : 



1. The refractive bodies are, in the main, composed of glycogen, surrounded 

 by a membrane of less soluble carbohydrate. 



2. During starvation the refractive bodies decrease in size, the glycogen dis- 

 appearing, until finally nothing but the membrane remains. 



3. If now the animal be fed with carbohydrate food (starch, glucoside, glyco" 

 gen, cellulose) glycogen is stored in the refractive bodies and they increase in 

 size. 



4. Proteids, gelatin, and fats cause no change in the refractive bodies, 



although the injested food particles are gradually dissolved. 



Frank W. Bancroft. 

 University of California. 



„ „ ,„,,.„, , „ , „ „. Both botanists and zoologists will be 

 Senn, 0. I'lagellata in Engler and Prantl " Die ° 



Naturlichen Pflanzenfamilien." I Theil, i interested in Dr. Senn's able mono- 

 Abth Lief. 202, 203, pp. 93-192, 1900. Leip- j.^ ^f j^is borderland group of organ- 



zig. W. Englemann. or- & r- & 



isms. The following orders are in- 

 cluded : Panto stomatijiece, Protoviastiginecz, DistomatinecB, Chrysomonadinece, Cryp- 

 tomonadinecB^ Chloro7notiadine(e, and Eiigletihiece. The work includes a compre- 

 hensive biological discussion of the group and keys to the genera, with very full 

 descriptions. Abundant illustrations serve to characterize many of the species. 

 Bibliographies are also very complete. Investigations since the publication of 

 the monographs of Biitschli and of Klebs have greatly increased the number of 

 known flagellates so that this revision of the group was much needed and will be 

 welcomed by all who have to deal with these widely distributed and biologically 

 important organisms. c. a. k. 



Johnston, J. B. A Sealing Stone Jar for Zoo- Stone jars eight to twenty-four inches 

 logical Laboratories. Amer. Nat. 34: 969- -^^ j^gj^^ ^^^ jg^ or twelve in diameter 

 971, 1900. * 



are made by the Zanesville Stoneware 



Co., Zanesville, O. The rim of the jar bears a groove to be filled with the seal- 

 ing fluid. A dependent flange on the lower surface of the lid fits into the groove, 

 thus sealing the jar. The edge of the lid projects so as to protect the rim of the 

 jar from dust. For daily class use water may be used as a sealing fluid, while 

 for permanent storage a very heavy paraffin oil is necessary. Lighter oils 

 or glycerin do not make good sealing fluids. The moderate cost, large storage 

 capacity, slight risk of breakage, the large mouth, and above all the ease of 

 opening and resealing make this an ideal storage jar for laboratories and museums. 



c. a. k. 



