1268 Journal of Applied Microscopy 



Medical Notes. 



Robin, A. Preservation of Sputum for Micro- The author experimented with carbolic 

 scopic Examination. Jour. Bost. Soc. Med. acid 5 per cent, solution, trikresol 2 

 Sci 5 : 7 



per cent., formaldehyde 5 per cent., 



and hydrochloric acid 10 per cent, to determine their preservative power on 

 sputum containing tubercle bacilli. The sputum treated was examined at the 

 end of 24 to 48 hours, after which time, weekly and then monthly examinations 

 were made for a period of four months. Except with HCl the preservation 

 was good and the bacilli stained deeply ; HCI seemed to disorganize the bacilli. 

 The author recommends the addition of an equal volume of a 5 per cent, solution 

 of carbolic acid to the sputum, which should be vigorously shaken in the bottle, 

 so as to break up the lumpy coagulation. c. w. j. 



Conn, H. W. How can Bacteria be Satisfac- I" answer to this question the author 

 torily Preserved for Museum Specimens ? offers the following method : A two 

 Jour. Bost. Soc. Med. Sci. 5: 7. ^ ,^ ,. . , , 



per cent, agar culture medium is placed 



in large test-tubes which are tilted so as to make agar slants. The tubes 

 are left undisturbed for six to eight weeks to allow the surplus moisture to 

 evaporate. They are then inoculated in long streaks and immediately sealed 

 wath plaster of Paris and paraffin. The cultures grow for a few days, then cease 

 growing and remain unaltered indefinitely. Only one unsatisfactory feature 

 presents itself; viz., moisture within the tube condenses on the inside of the 

 tube with changes of temperature, thus rendering the tube cloudy and for the 

 time injuring the value of the display specimen. c. w. j. 



Eastes, 0. L. Note on the Phenyl-Hydrazin P^ace 60 c. c. of filtered urine in a 

 Test for Sugar. Brit. Med. Jour., Feb. 23, beaker of 100 c. c. capacity, add 1 gm. 

 ^ ■ of sodium acetate, and a little less of 



phenyl-hydrazin hydrochlorate. Stir with glass rod, which is left in the mixture 

 throughout the operation. Place beaker on water bath and allow the mixture to 

 evaporate gradually down to 10 or 15 c. c, occasionally scraping the sediment 

 from the sides of the beaker if such tends to collect. When reduced to the bulk 

 indicated, remove flame and allow the liquid to cool. When quite cool examine 

 under microscope. Ozazone crystals will have formed if there is one part per 

 thousand or more of sugar in the urine. If no crystals are formed it may be 

 safe to conclude that no sugar (glucose) is present. c. w. j. 



Uhlenhuth. Method for the Differentiation of If, at intervals of six to eight days, 



the Blood of Various Animals with especial gn^^U amounts of the defibrinated blood 



Reference to the Demonstration of Human 



Blood. Deutsche Med. Wochenschr., Feb. of any animal is injected into the rabbit, 



7' '90I- changes are produced in the rabbit's 



blood which cause it to give a reaction with the blood of that other animal alone 

 and with no other. If a few drops of the serum of a rabbit that has been treated 

 with ox blood, for example, are dropped into each of a row of test-tubes contain- 

 ing dilute solutions of the blood of various animals, absolutely no reaction is 

 produced in any tube except that containing ox blood, which at once shows a 

 slight turbidity, which increases on standing and finally develops into a floccu- 

 lent precipitate. 



The blood of a rabbit which has been injected with human blood, furnishes 

 an infallible reagent for detecting human blood even in very small amounts, and 

 after having been allowed to dry for four weeks. c. w. j. 



