1334 Journal of Applied Microscopy 



dates show that the young and actively growing plants were so strongly polarized 

 as to resume the original manner of growth ; that only older, more slowly grow- 

 ing forms succumbed to the external conditions, and turned root into shoot and 

 shoot into root. These results agree with those of Winkler of an earlier date. 



Noll takes exception to the definition of " Energid " as given by Sachs, and 

 calls the Siphoneae " single but multinucleate energids," laying stress upon the 

 " Hautschicht " rather than upon the nucleus and its dominated mass of proto- 

 plasm. He therefore defines the energid as a " one or many nucleate plasmatic 

 body enclosed in a definite wall." Philip G. Wrightson. 



Chicago. 



Life, A. C. The tuber-like rootlets of Cycas The coral-like outgrowths on the up- 

 revoluta. Bot. Gaz. 31 : 265-271, lo figures ward rising roots of Cjras revolida 

 ' ' have long been known, and the endo- 



phytic alga and fungus have also been described. Life has made a careful study of 

 the subject from thin microtome sections and has been able to give a more precise 

 account. In regard to the reputed dichotomy, he finds that not all of the meri- 

 stem passes over into the two branches, but that a small portion is left as a 

 bridge between them. This small portion, however, does not continue the main 

 axis, and very soon disappears so that sections of roots in which the branching 

 can be seen at the surface show no trace of meristem between the two branches. 

 The development of the algal zone is clearly figured and described. Three 

 forms of fungi were observed. They make their appearance in advance of the 

 algal zone and seem to prepare the way for the algae, which are referred to the 

 genus Nostoc. The presence of the fungi affects the intercellular spaces so that 

 they become the rather large chambers occupied by the algae. The Nostoc 

 probably enters through the numerous lenticular areas. It is suggested that 

 the tubercles serve for aerating and also assist in nitrogen assimilation. 



c. J. c. 



CYTOLOGY, EMBRYOLOGY, 



AND 



MICROSCOPICAL METHODS. 



Agnes M. Claypole. 



Separates of papers and books on animal biology should be sent for review to 



Agnes M. Claypole, Sage College, 



Ithaca, N, Y. 



CURRENT LITERATURE. 



^ _, „ „ ,. , ,. . ^ , , The work gives the results of studies 



Overton, h. Studien ueber die Aufnahme der ° 



Anilinfarben durchdielebendeZelle. Prings- upon the action of anilin Stains on 

 heim's Jahrb.f.wiss. Bot. Bd. 34: 669-701, animal and plant cells. Basic anilin 

 1899. 



stains are readily taken up by both 



kinds of cells. Four classes of these stains were studied in detail. (1) 

 Triphenylenethane stains : rosanilin (chlorhydrate, nitrate, sulfate), gentian violet, 

 methyl violet, dahlia, anilin blue soluble in alcohol, toluidin blue, victoria blue, 

 malachite green, methyl green, iodine green, auramin, rhodamin ; (2) CJmionhnid 



