1336 Journal of Applied Microscopy 



trated solution of bismarck brown in one per cent, acetic acid, or in lithium or 

 borax carmin for 20-30 minutes ; in case of using borax carmin it should be 

 washed out in hydrochloric acid alcohol. Washing with water follows. (3) 

 Stain next for 10-15 minutes with 20 per cent, aqueous solution of malachite 

 green, also brilliant green or victoria green. The solution is made by diluting 

 the alcoholic solution five times. Wash out in water. (4) Stain for i-1 minute 

 long according to Van Gieson's tincture method or with concentrated aqueous 

 picric acid solution, which is diluted 4-5 times with water. Wash in water. (5) 

 The quickest possible dehydration and decolorization in absolute alcohol, mount- 

 ing in xylol and balsam. Turpentine or bergamot oil may be used in the place of 

 xylol. All the decolorizing necessary is easily managed, and can be allowed to con- 

 tinue for a long time. In preparations made in this way the beryl-green corpus- 

 cles are distinguished from all other structures, which are a gold-brown from 

 bismarck brown or red-gold from carmin. a. m. c. 



Godlewski, E. O. rozmnazanin jader w nies- I" O^'^er to learn to recognize the 

 niach prazkowanych zwierzat kregowych multiplication of nuclei in Striated 

 (Ueber Kernvermehrung in den querges- , ^ , , . , 



treiften Muskelfasern der Wirbelthiere.), muscle of vertebrates durmg embryonic 

 Bull, de I'Acad. des Sci. de Cracovie, Avril, and postembryonic development, the 



author studied the striated muscle of 

 newly born guinea pigs and mice, also those of salamander larvae. The extrem- 

 ities of embryos taken from the mother or of narcotized newly born young were 

 put in toto into the fixation fluid. Perenyi's fluid or concentrated sublimate 

 solution with the addition of two per cent, acetic acid was used, followed by 

 increasing strengths of alcohol. After hardening, small pieces of muscle were 

 separated from the bone. During these fixing and hardening processes a great 

 deal of contraction takes place in the muscle fibers. Muscles were cut in 

 paraffin in longitudinal, transverse, and oblique sections five yu in thickness. 

 These were stained in thionin, also in Heidenhain's haematoxylin, double 

 stained with bordeaux red or eosin. In preparations so made the nucleoli are 

 sharply tinted with red, so that a clear contrast is obtained between these and 

 the blue chromatin bodies. a. m. c. 



His, W. Ueber Sogenannte Amitosis. Anat. Since the discovery and demonstration 

 Anz. Centralblt. f. d. Gesam. Wiss. Anat. of bipolar mitosis it has been known 

 18: 52-60, iQoo. , 1 ^ • , • , , 



that nuclear figures exist which do not 



agree with the newly discovered principles. Flemming gives a second type of 

 division, direct or amitotic. The characters of this kind are negative, absence 

 of the spindle and splitting of the chromosomes. This form was considered 

 degenerate or pathological, but recent work shows that by changing the con- 

 ditions of growth the cells of Spirogyra may be made to pass from mitotic 

 division to amitotic and back again, without disturbing the normal conditions of 

 growth. This places the difference in the two types of division in the province 

 of physiology and the problem is to determine in how far the two processes follow 

 a common law, and in what way they are related to each other. It has already 

 been suggested (His) that amitosis may be plenipolar mitosis and be related 

 to the growth of multinuclear giant cells and syncytial formation. For these 

 processes His suggests the name " syncaryosis." 



