1338 Journal of Applied Microscopy 



dehydrated in ninety-eight per cent, alcohol, cleared in origanum oil, and mounted 

 in balsam. All preformed hyaline cartilage shows itself distinct microscopically, 

 through its blue violet color, from the rest of the brownish red tissue. Micro- 

 scopic investigation shows that the blue color has its place in the ground sub- 

 stance of the cartilage. This blue cartilage network makes a strong contrast with 

 the red nuclei of the merely light blue or non-colored cartilage cells. In the embry- 

 onic fibro-cartilage of the intervertebral discs, as yet undifferentiated, the central 

 cartilage cells stain blue, the nuclei red. The cells always become paler toward 

 the margin. With orcein, embryonic elastic cartilage gives no double stain. 

 The change in the color from that of adult cartilage is worth mention. 

 Here the ground substance is reddish, the cartilage cell with its surrounding 

 area intense blue, so that the red nuclei can only be seen in the thinnest sec- 

 tions. Similarly changed is the adult fibro-cartilage ; only a few fibro-bundles 

 are blue. The elastic cartilage also shows no double stain in the adult condi- 

 tion. Developed bone, both decalcified and non-decalcified, likewise shows no 

 double stain. e. j. c. 



Linser, P. Ueberden Bau und die Entwicklung The Weigert method was used to dem- 

 des Elastischen Gewebes in der Lung. ^ . i ,• ,• • ,i i 



Anat. Hefte. H. 42, 43: 307-336, m. 3 Tfln , onstrate the elastic tissue in the lung. 



1900- The stain acted usually in 2-3 hours, 



yet a longer time for staining, even to 24 hours did not do much harm. If a 

 shorter time was used, 15-20 minutes, no usable results could be obtained. By 

 the longer staining one had this advantage, with others, to stain the adjoining 

 kinds of connective tissue in contrast. Usually a simple washing out in strong 

 alcohol sufficed to make the bundles appear separate. After a longer continu- 

 ance of the staining process it is necessary to differentiate in hydrochloric acid 

 alcohol if one wishes to have the elastic fibers stained. For a nuclear stain, 

 alcoholic borax carmin and lithium carmin were used ; control-stains were 

 carried on with haemalum-eosin after Van Gieson's method. For investigation 

 12 embryos, 3.3 cm. long (Kopf Steiss), to the oldest fcetal stages, were used. 

 Further, fourteen children up to five years of age and eight older human lungs 

 of different ages. Further, eight different stages of the rat, both before and after 

 birth, lungs of young and old cattle, of new born and older guinea pigs, of hare, 

 dog, horse, pig, roe, stag. Tissues were preserved in formalin or alcohol and 

 imbedded in celloidin. e. j. c. 



Foote, K,and Strobell, E. C. Egg of Allolo- A series of micro-photographs of this 

 bophora foetida. Journ. of Morph. 16: egg is published to illustrate the fol- 

 07- I , 3ps., 1900. lowing points: (1) The effect on the 



cytoplasm of the different fixation fluids now in common use. (2) The charac- 

 ter of the fertilization cone. (3) The position of the middle piece of the male 

 aster. (4) The origin of the sperm granules. (5) The early stages in the 

 development of the pronuclei. (6) The presence of osmophile granules in the 

 nucleoli of the germinal muscles. The photographs have been taken at two 

 magnifications, G60 and 050, and it is believed that proof has been offered of 

 some of Foote's earlier conclusions, in regard to the cytoplasmic origin of centro- 

 some of the male aster. . a. m. c. 



