138:2 Journal of Applied Microscopy 



The first division of the nucleus of the embryo-sac in which the reduction in 

 the number of chromosomes is effected, takes place after the opening of the 

 flower. The number of chromosomes in the gametophyte is twelve, but in one 

 case six were counted. The antipodal nuclei become fragmented into a varying 

 number of pieces. The generative cell of the pollen grain often occupies the 

 greater portion of the space within the spore, and has an unusually thick mem- 

 brane. The vegetative nucleus remains in the end of the tube after the two 

 male nuclei have been discharged into the sac. One of the male nuclei conju- 

 gates with the nucleus of the egg, and the other becomes applied to the upper 

 polar nucleus, so that the definitive nucleus results from the fusion of three 

 nuclei, the two polar nuclei and one of the male nuclei. 



After fertilization, the egg gives rise to an irregular mass of cells, in which 

 the beginnings of several embryos may be distinguished. The polyembryony of 

 Tulipa is very much like that described by Jeffrey for Erythronium. 



c. J. c. 



CYTOLOGY, EMBRYOLOGY, 



AND 



MICROSCOPICAL METHODS. 



Agnes M. Claypole, Cornel] University. 



Separates of papers and books on animal biology should be sent for review to 



Agnes M. Claypole, 125 N. Marengo avenue, 



Pasadena, Cal. 



CURRENT LITERATURE. 



Doflein, F. Cell Division in Protozoa. Zool. Dr. F. Doflein has Studied Noctiliua 



Jahrb. 14 : 1-16, 1900. E.xtracts from miliar is with especial reference to the 



Royal Mic. Jour. April, iqoo. , , . , 



■' f ' ^ nuclear changes accompanying cell 



division. The life cycle is as follows : Afterdividingrepeatedly the adult comes 

 to rest, copulation of two individuals occurs, followed by rapid budding. The 

 liberated buds are at first similar to Dinoflagellata, but ultimately become con 

 verted into adults. Division occurs, a sphere appears near the nucleus, and 

 a process takes place believed by the author to have a superficial resem- 

 blance to metazoan karyokinesis. The division of the nucleus appears to be 

 in some degree independent of the division of the sphere, the division of the 

 latter being closely associated with plasmic division; the author believes that the 

 former structure is but a concentration of the plasma itself, hence the close 

 relation. The budding after copulation consists of a rapid cell division during 

 which the division products remain united by a common stroma. It is uncertain 

 whether this stroma indicates a reduction process or not. A discussion on the 

 structure of protoplasm and the causation of its movement is also given in the 

 paper. a. m. c. 



The authors use the following simple 

 Stephens, J. W. W. and Christopher, R. S. R. ., j r 1 4^ • • c, 



Technique for Malaria Blood. Roy. Soc. method for preparmg and stammg films 

 Report to Malaria Comm. od Series, 1900. of malaria blood : The finger is pricked 

 Ext. from Royal Mic. Jour., April, igoo. ■, . ■ , ■ , ,, 



■' J 1 f ■> J ^\m a triangular surgical needle 



and a clean glass slide touched to the exuding blood. The drop thus obtained 



