1422 Journal of Applied Microscopy 



cells. If this theory be true we have a measure of the degree of productive cell 

 activity in the appearance and still more in the number of the mononuclear 

 leucocytes and lymphocytes in the pus, and hence a point of diagnostic value in 

 determining the stage of the inflammation. Especially in gonorrhtea the finding 

 of many gonococci, eosinophiles and few lymphocytes would speak for an acute 

 infection, while the finding of few cocci and abundant mononuclear cells would 

 indicate an exacerbation of a chronic process. j. h. p. 



„.„„._ »;r . . ir o • • This method is based on the fact that 



Harris, H. r. A rsew Method of htaining 



Elastic Tissue. Proceedings of the Patho- haematin solutions have a remarkable 

 logical Society of Philadelphia, 4: 167-16S, affinity for elastin. Harris directs that 

 1 90 1 . \ 



the stain shall be prepared as follows : 



Haematoxylin, 0.2 gm. ; aluminum chloride, U.l gm. ; .50 per cent, alcohol, 100 

 c.c. Dissolve the haematoxylin and aluminum chloride, and then carefully heat 

 the solution to the boiling point ; 0.6 gm. of mercuric acid is now slowly added, 

 and as soon as the mixture assumes a dark purple color it is removed from the 

 flame and cooled rapidly. The stain is filtered and one drop of hydrochloric 

 acid is added. The stain requires several weeks to ripen. It appears to keep 

 indefinitely. 



Sections are stained from five to ten minutes and are then washed for about 

 a minute in a 1 per cent, solution of nitric acid in alcohol ; the acid alcohol is 

 then thoroughly removed with pure alcohol, and the sections are cleared and 

 mounted. j. H. p. 



Howard, W. T. Observations on the Character In 1898, Councilman published his 

 of the Cells in the Exudation in Acute Inter- , , , . , .-,• 1 1 •^' 



stitial Nephritis, with Special Reference to ^tudy of acute mterstltial nephritis, 



the Presence of Cells with Eosinophilic He showed that the disease is charac- 



granulations. American Journal of the , • j i_ 1 11 1 • ci^ ^- 



Medical Sciences, 121 : 1 51-163, 1901. terized by general and local infiltration 



of the interstitial tissue of the kidney 

 with Unna's plasma cells. He agreed with Marschalko that these cells are 

 derived from lymphocytes, and stated that they are carried to the kidney in the 

 blood current. In addition to the plasma cells Councilman found a variable 

 number of lymphocytes and polynuclear leucocytes in the exudation. 



Howard has confirmed Councilman's observations, and in addition found 

 large numbers of typical eosinophilic leucocytes in the interstitial exudation and 

 in the blood vessels in the three cases of the disease which he has studied. The 

 eosinophilic leucocytes are for the most part brought to the kidney by the blood- 

 vessels and reach the interstitial tissue by emigration, but they may be formed 

 locally from plasma cells. j. h. p. 



Fuchs, E. Beitrage zur Kenntniss der Entste- F"chs holds that the eosinophilic cells 

 hung, des Vorkommens und der Bedeutung have no single mode of origin. Eosin- 

 •' eosinophiler " Zellen, mit besonderer , ... , , , j .. r 



Berucksichtigung des Sputums. Deutsches oP^iliC granules can be formed out of 

 Archiv fiir klinische Medicin, 63: 427-443, neutrophilic granules, or from frag- 

 ^^' ments of broken down red blood cor- 



puscles, which when ingested by cells transform the cells into eosinophiles. 

 This probably explains the increase of eosinophiles in various haemorrhagic pro- 

 cesses. Eosinophiles can be formed in all the tissues. They are especially 



