1468 Journal of Applied Microscopy 



The eggs of Culex may be had with ease by exposing a bucket of water out 

 of doors in a mosquito locality on almost any summer night. If the egg masses 

 be transferred from the bucket to the prepared breeding-jar, the growth of the 

 larvae can be watched, and their transformations can be observed with perfect 

 ease. Occasional specimens can be taken out and preserved, to illustrate 

 variations of different stages of growth. Accurate notes can be kept as to 

 temperature, periods of transformation, and so on. A series of dates, provided 

 several jars are under observation, can be written from time to time upon a slip 

 of paper, which may be pinned to the edges of the cloth covering of each jar. 



Where the eggs of Anopheles, for example, have not been found, females 

 collected at large may be liberated in such a prepared breeding-jar. They will 

 rest on the under side of the cloth covering during the day, and at night will lay 

 their eggs on the surface of the water. It is desirable to have a stick in the 

 water, or a leaf, or a bit of cork floating on the surface. I have had no difficulty 

 in obtaining the eggs of Anopheles in large numbers in this way, and the eggs 

 of Culex as well, but although as many as fifty females of Psorophora have been 

 liberated in breeding-jars prepared in this way, I have not been able to get the 

 eggs of this genus, which, as a matter of fact, are yet unknown. It is possible 

 that Psorophora does not deposit its eggs upon the surface of water. This, how- 

 ever, is unlikely, and it is rather to be supposed that the females used in my 

 experiments were not old enough for oviposition, and died from the confinement 

 of the jar before the egg-laying period arrived. 



When one wishes to study closely the movements and intimate habits of the 

 early stages of mosquitoes, a great deal may be observed through the glass sides 

 of the jar, by using a coarse lens and studying those near the side, but when a 

 closer study is desired, individual larvae or pupae may be lifted out with a strainer 

 and put in a shallow porcelain vessel, where they can be watched with ease under 

 a dissecting microscope. Anopheles larvae may be studied in this way very 

 easily, and no nature study could be of more fascinating interest than the obser- 

 vation of these creatures, lying as they do with the body practically in a single 

 plane, so that they may be easily watched, with the mouth parts in constant 

 action, and the head occasionally turning upside down, and the reverse, with 

 lightning-like rapidity." c. a. k. 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



Joseph H. Pratt. 



Harvard University Medical School, Boston, Mass., to whom all books and 

 papers on these subjects should be sent for review. 



Sailer, J. Primary Endothelioma of the Left At the orifice of the left superior pul- 

 Superior Pulmonary Vein. Contributions . , , ^ 



from the William Pepper Laboratory of monary vem there was a dense mass of 

 Clinical Medicine. Philadelphia, pp. 416- fibrous tissue, almost occluding the 

 444, 1900. , , ,. , . , 



lumen, and extendmg on the auricular 



wall to the upper end of the left inferior pulmonary vein. The wall of the 

 superior pulmonary vein was nearly uniformly thickened throughout its whole 

 course in the upper lobe, forming a round cord fifteen mm. in diameter. Upon 

 section it was seen to be made up of grayish and yellowish tissue. The upper 

 lobe was contracted, pigmented and airless. 



There was found, on microscopical examination of the thickened vein, hyper- 

 plasia of the connective tissue stroma and enlargement of the lymphatic spaces 



