1488 



Journal of Applied Microscopy 



After using the various developers put on the market, with various degrees of 

 success, I have at last struck upon one which fulfills all of my requirements. 

 This salt goes by the name of Ortol, and is purchased in sealed tubes with an 

 accompanying cartridge of the required amount of soda. The contents of each 

 are dissolved in 20 oz, of water and kept in separate bottles. 



Various grades of intensity may be gotten by regulating the strength of the 

 solution and by the use of more or less soda as occasion requires. In Fig. 1 the 

 development was carried slightly too far, but shows great contrast and clearness. 

 In the second illustration we have a transverse section of the human sciatic nerve 

 developed for detail rather than contrast. 



Fig. 2. — Transverse section of human sciatic nerve. 



The following proportions may be varied somewhat according to exposure, 

 but I have found them to answer for all purposes by using more or less bromide : 

 For negatives : 



Ortol, - - - - 2 parts stock solution, 



Soda, . - . . 2 " " 



Water, . - - - 4 " 

 For lantern slides : 



Ortol, - - - - 2 parts stock solution. 



Soda, - - - - 1 part " " 



Water, - - - - .3 parts. 

 For bromide papers : 



Ortol, - . - - 1 part stock solution, 



Soda, ... - 1 " '< 



Water, - - - - 8 parts. 



I do not believe any definite rule can be set down as to just how much 



