1917] 



on The Treatment of War Wounds 



31 



The Body Fluids. 



Let me begin with the blood fluids, and let me take you directly 

 to the following experiment. I call it the experiment of pijo-sero- 

 cuUure — i.e. the experiment in which we implant pus into serum to 

 see which of the microbes of the wound can grow in the blood fluids. 



We procure for our experiment a suppurating wound. We take 



Fig. 1.— Method of pyo-sero-culture.—A, Pipette which has been 

 implanted by the wet-wall method, and has then been filled in by the 

 wash and after-wash procedure with unit-volumes of serum. By the 

 side of the pipette to the right is ranged a series of drops representing 

 the series of unit-volumes of serum blown out in order from the 

 pipette, and, finally, to the right of the drops is a series of lines 

 representing linear implantations made upon agar. B, Eesults of the 

 series of linear implantations made with the unit- volumes of the 

 patient's serum. C, Results of the series of linear implantations made 

 with the unit-volumes of the normal serum which was used as a 

 control. 



from it a specimen of pus containing a large variety of different 

 organisms. At the same time we take from the patient's finger a 

 sample of blood, and we take a specimen also of our own. When 

 the serum has issued from the clot we take a capillary pipette, fit 

 a rubber teat to the barrel, and inscribe a mark upon the stem at 



