163 



the purified water. This is the precise change we observed in 

 Cochituate water. 



I object to the notion that this rich green chlorophyl (a spe- 

 cimen of which Dr. Jackson exhibited) is the result of mixtures 

 of different colored oils from cyclopeans ! Such a result is 

 impossible I 



Berzelius, in his Traite, tome VI. p. 504-5, French ed., 1850, 

 states, that the sp. gr. of myrica wax is 1.015, which will account 

 for the fact that we find it in the water, near the bottom of the 

 lake. 



That myrica wax yields oil soluble in alcohol, and that this 

 oil contains oleine and stearine, Berzelius also states, p. 505, 

 " By cold alcohol, we extract oleine from the wax of myrica." 

 " Boiling alcohol dissolves it in small quantity ; the solution, by 

 cooling, deposits stearine.'" " It deposits stearine in small, color- 

 less scales, while the solution of oleine remains colored green 

 by the whitened wax of myrica." 



Thus, we find myrica wax yields oil to the very solvent em- 

 ployed by Dr. Hayes, and which was also used by me in some 

 of my experiments. Nothing has yet come to prove this oil, 

 found in Cochituate water, to be other than the oil usually found 

 with the wax of myrica, and of green leaves colored by chloro- 

 phyl, which is generally accompanied by this fat vegetable 

 matter, consisting of a mixture of wax and oil. 



I see no reason to doubt that the oil found in the structure of 

 t^iese cyclopeans came from the same source, and until proofs 

 are presented of its derivation from fishes, I shall adhere to my 

 original opinions. 



Dr. Bacon observed, that as to the question of the animal or 

 vegetable nature of the oil which Dr. Hayes had found, and 

 which he had himself directly extracted from the bodies of the 

 crustaceS, it should clearly be considered animal. The oil, as 

 seen under the microscope, was not contained in the alimentary 

 canal, but in the tissues external to it, and beneath the carapace. 

 Whatever the original source of the oil might have been, it had 

 become assimilated in the animal, and was analogous to the 

 adipose tissue of other animals. As to the objection that no oil 

 in the lake rises to the surface. Dr. Bacon observed, that the oil 



