NO. 2$ INFLUENCE OF ATMOSPHERE ON HEALTH 35 



boxes were cleaned out daily and the animals were well fed. They 

 not only must have inhaled each other's breath, but must have 

 eaten food contaminated with each other's fur and excreta. After 

 so living for weeks, we injected rat's serum into the guinea-pigs 

 and guinea-pig's serum into the rats. In neither case was there 

 any evidence of anaphylaxis. 



We made the injections into the veins of the ear of most of the 

 guinea-pigs, using a fine needle. In others, and in the rats, we 

 made the injections into the heart. A month later we gave a second 

 dose of rat's serum to the guinea-pigs, and each one died within a 

 minute or so with all the symptoms of anaphylactic shock. The 

 rats, on the other hand, did not become sensitized to guinea-pig's 

 serum. It is recognized that rats are immune to anaphylaxis. 



We sensitized more guinea-pigs by an injection of rat's serum and 

 a month later injected into these the condensation water we obtained 

 from the air drawn through a vessel containing rats — a father, 

 mother and seven young rats. The result was nil. 0.5 and 0.75 cc. 

 were the doses of condensation water injected. While entirely 

 unaffected by the condensation water, these sensitised guinea-pigs 

 suffered from anaphylactic shock when a dose of rat's serum 1 of 

 the minutest volume) was injected three hours later. We are 

 driven to conclude, then, that Amoss and Rosenau's positive results 

 were due to contamination of the condensation water by saliva, and 

 that when the experiments are conducted under the conditions which 

 pertain in a crowded room, there is no evidence of the exhalation of 

 any volatile protein. 



Brown-Sequard and D'Arsonval 3 sought to substantiate their re- 

 sults obtained with condensation water by another form of experi- 

 ment. They confined a rabbit in each of a series of four metallic 

 cages connected by means of rubber tubing through which a con- 

 tinuous current of air was aspirated. The animal in the last cage 

 breathes the air which has passed through all the previous cages 

 and which is contaminated with the other rabbits' breath. This 

 animal after a time dies, then after a time the rabbit in cage 3 

 succumbs, while rabbits in cages 1 and 2 usually survive. They 

 could not attribute the deaths to CCX poisoning, because they rarely 

 found in the last cage more than 3 per cent with small animals, and 

 6 per cent with larger animals. On interposing between the last two 

 cages (3 and 4) absorption tubes containing concentrated 1LS<),, 



'Compt. Rend. Acad, de Sc. Paris, 1889, Vol. 108, 267-272. 



