40 



SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. 60 



End of 

 first week 



(I) 



Chamber II, guinea-pig, weight in grams. ..292 325.7 

 Percentage of CO; : 2. 95-3 . 78 



(II) 

 Chamber II, rats (3) weight in grams. .. .1 90 96 



2 81 



3 78 



90.7 

 80.5 



Percentage of C0 2 |2. 21-2. 59 



End of 

 second 

 week 



370.5 



3-88 



End of 

 third 

 week 



385 

 3.76 



End of 

 fourth 

 week 



III .2 128.7! 144 

 99.5J IO9.5; 120.5 

 84.8 85.2! 85.5 



2.88 2.55 I 2.48 



We (M. F. and L. H.) also carried out experiments of a some- 

 what different kind, the results of which wholly negative the Brown- 

 Sequard and D'Arsonval statements. 



We confined tame rats and guinea-pigs together in a deep wooden 

 box, the lid of which was so shut down as to give imperfect ventila- 

 tion. The animals lived for 2^ weeks in an atmosphere containing 

 1.6 to 3.8 per cent C0 2 . 



Guinea-pigs 



(1) Large, short hailed, 



brown 



(2) Large, black, short 



hair 



(3) White dot and black. 



(4) White dot and brown 



fur 



(5) Large, long hair 



(6) Right black and left 



brown 



Rats 



Stumpy tail 



Black+ 



Black— 



Red head 



Warty + 



Warty— 



Weights 



Feb. 1 

 Initial 

 weights 



Grams 

 335-5 



33i 

 I9I-5 



139-5 

 266 



133-5 



165 



186.5 



126.5 



190 



166 



Feb. 8 



Incre- 

 ment 

 first 

 week 



Grams Grams 



381.5 



373 

 229.5 



176 

 3!6-5 



46 



42 

 38 



36.5 

 50 



251.5 ! 60.5 



159-5 

 182.5 

 212.5 



153-5 

 224 



190.5 



17-5 

 26 



27 

 34 

 24-5 



Feb. 15 



Grams 

 413 



406 

 263 



203.5 



34o 



278.5 



163.5 



234 



197-5 



Incre- 

 ment 



second 

 week 



Grams 

 3'-5 



33 

 33-5 



27-5 

 24 



2-5 



i-5 



—•5 



Ventilation 



> too little 



first day 



^Second 

 week 



In the third week the lid 

 of the box was accidentally 

 closed and left so all night. 

 Three guinea-pigs and one 

 rat died from the effects of 

 suffocation. The remain- 

 der lost weight very seri- 

 ously; for example, guinea- 

 pigs 42 to 77 grams and rats 

 3 to 26 grams. The loss of 

 weight was recovered at the 

 end of another week, dur- 

 ing which the box lid was 

 left full open. 



The fact that the animals put on so much weight shows con- 

 clusively that there was no active toxic substance in the exhaled air ; 

 the experiment negatives the conclusions not only of Brown-Sequard 



