6 THE COMPOSITION OF EXPIRED AIR, 



ill the axilla, five out of seven rabbits ditiil rapidly. The results were much the 

 same as when it was injected into the blood. The quantity of the condensed liquid 

 injected in these seven was : 20 c. c. in one case, 25 c. c. in three cases, 31 c. c. in 

 one case, 4U c. c. in one case, and 44 c. c. in another case. After death, consider- 

 able congestion of the viscera was noted, especially of the lungs. No ai)pearanoe 

 of embolism was noted. The brains and its membranes were congested, but with- 

 out visible lesion. The condensed liquid turns concentrated sulphuric acid yellow. 

 The poison is reduced by ammoniacal nitrate of silver solution as well as by chloride 

 of gold. After boiling in a close vessel it is still toxic, showing that the poi.son is 

 not a micro-ortranism. The boiled lung liquid poisons with more rapidity than that 

 which hiis not been sterilized, and may kill a pigeon and a guinea-pig as well as a 

 rabbit ; it may kill by being injected into the rectum or into the stomach ; a 

 fruincainfr two months old was killed within twelve hours by an injection of 3 c. c. 

 into the peritoneal cavity. If injected into the lungs this liquid jtroduces rapid 

 congestion followed by true inflammation and red hepatization. 



In an e.xperimeut with two dogs it was arranged that one breathed ordinary 

 air and the second inhaled air which came from the lungs of the other. The dogs 

 were of the same weight, 15 kilograms. The experiment continued for six hours 

 and forty minutes. No appreciable or immediate consecutive accidents were 



produced. 



In a second experiment the pulmonary liquid was collected from dogs through 

 a tracheotomy tube to exclude impurities furnished by the mouth. The air inhaled 

 was first washed to remove dust. The moisture in the air expired was condensed, 

 and the liipiid collected in a flask surrounded by ice. At the moment of injection 

 this li(]uid was filtered, and was then injected at the temperature of the laboratory, 

 about 12° C. If the animal was kept immovable from 12 to Ui hours, inflammation 

 of the air passages was produced. The liquid of the first hours came from a 

 thoroughly sound lung, and in the later houi-s from a diseased lung. The two 

 were collected separately and tried separately. For one kilogram of the animal, 

 for each hour, the mean quantity of fluid obtained was 0.38 grammes, varying 

 from 0.28 to 0.48 grammes. It was greater in the beginning and lessened the longer 

 the animal was kept in a fixed position. It was injected into the marginal vein of 

 the ear of a rabbit by means of a syringe, 75 c. c. being injected. When the injec- 

 tion <lid not exceed 40 to 50 c. c. the time occupied by the injection was from G to 

 15 minutes. Experiments made by injections upon the dog were negative with- 

 out exception. Experiments maile upon the rabbit produced lesions, but the 

 relation between these ami the injections was uncertain. 



Dastre and Loye, in 1888, (18), reported that they had exposed one dog to 



