A^^^ its effects upon a>tmal life. 7 



the expired breath of another for six houi-s without noting any effects. They 

 inoculated animals with the condensed moisture of respiration, as follows : 



5 rabbits, each ^;} to 75 c. c. of the fluid. Resuhs negative. 



2 guinea-pigs, " 5 " 7 " " 



2 dogs, " 3° " 53 " " 



2 frogs, " 2 " 3 " 



2 rabbits, " 50 " 190 " " " Died. 



A young dog, 30 " of water. " 



They found that 50 to 70 c. c. of the condensed fluid of I'espii'ation (20 to 35 

 c. c. per kilo.) could be injected into the veins of the ear of a dog without producing 

 any of the symptoms reported by Brown-Sequard and d'Arsonval. They observed 

 one death dui'ing the injection of 190 c. c. (60 c. c. per kilo.), yet by control experi- 

 ments with water they obtained a more remarkable result — a rapid death from the 

 injection of 30 c. c. of distilled water (25 c. c. per kilo.). 



Russo-Giliberti and Alessi, in 1888, (19), reported experiments confirming the 

 results obtained by Dastre and Loye. 



Wiirtz, in 1888, (20), attempted to obtain the "ptomaine" of Brown-Sequard 

 and d'Arsonval from the fluid condensed from expiied air. By expiring through a 

 1 per cent, solution of oxalic acid he obtained, besides ammonia, a volatile organic 

 base which was precipitated by Bouchardet's reagent and by potassio-mercuric 

 iodide. With platinic chloride it formed a double salt, crystallizing in short needles, 

 and a soluble salt with auric chloride. When heated to 100 ° C. it gave off a 

 peculiar odor. This basic substance, he thought, might be regarded as a leuco- 

 maine. 



Brown-Sequard and d'Arsonval, in 1889, (21), reported a new form of experi- 

 ment by means of which they obtained additional evidence in support of their for- 

 mer statements. The new form of expeiiment consisted in confining animals 

 (rabbits) in a seiies of metallic cages connected by means of rubber tubing, through 

 which a constant current of air is aspirated. The animal in the last cage of the 

 series receives air that has traversed the entire series of cages, and is loaded with 

 the impurities from the lungs of the animals in the other cages. This animal suc- 

 cumbs, after a time, to the atmospheric conditions present. After another interval 

 of some hours, the animal in the next to the last cage also dies ; the first and second 

 animals usually remaining alive. They could not attribute the death of these 

 animals to excess of carbonic acid in the atmosphere of the cages, because they 

 rarely found more than 3 per cent, of this gas in the last jar with small animals, or 

 6 per cent. Avith larger animals. On placing absorption tubes containing concen- 

 trated Hg SO4 between the last two cages, the animal in the last cage remained 



