3G 



THE COMPOSITION OF EXPIRED AIR, 



and the ammonia in a like quantity of the same water was determined simultaneously and 

 deducted from the amount found in the diluted fluid. The minute quantities of ammonia found 

 in the fluid in some of these determinations required the greatest care in manipulation to avoid all 

 sources of contamination — in the collection of the fluid as well as subsequently in the distillation 

 and nesslerization. The greatest care had to be exercised, therefore, in cleansing all apparatus 

 used, and in the preparation of the different reagents. 



The fluid for the first seven determinations was collected from my own breath, and, for the 

 next thirteen determinations, from the breath of the man with the tracheal fistula. The remainder 

 of the determinations were made on the fluids collected from the breath of the consumptive. 



Table B. 



DETERMINATION OF FREE AND ALBU.MINOID AMMONIA. IN CONDENSED FLUID OF RESPIRATION. 



The amount of organic matters present in the condensed fluid, as shown by its reducing power 

 upon solution of permanganate of potash, is represented in Table C, the results being calculated 

 to Mg. of O. consumed to one litre of the condensed fluid. The table also shows the amount of 

 fluid used in each of the determinations and the time required to collect such amount. In three 

 of the experiments the amount of air expired is also given. These determinations were made 

 according to the methods now in common use for the determination of organic matter in water as 

 modified by Kubel ; the fluid being diluted with a definite amount of distilled water, the reducing 

 power on permanganate of which was simultaneously determined and deduced from the results 

 obtained. The ebullition of the fluid was always carefully timed — the time being five minutes. 



♦Merely a trace found. 



I 



