AND ITS EFFECTS UPON ANIMAL LIFE. 41 



Cover-slip preparations were made from the abscess, blood, lungs, liver, spleen, an.d kidney. 

 Those from the site of the abscess were the only ones showing any organisms ; numerous cocci, 

 with a few bacilli, were observed. 



Microscopic examination of the organs hardened in alcohol and mounted in celloidin : 'J'he 

 liver presented some increase of connective-tissue elements between the lobules. The whitish 

 bands on the surface of the organ, noted at the autopsy, were found to be due to this increase in 

 connective-tissue elements in the inter-lobular spaces. No change was noticed in the liver cells 

 themselves. .All the other organs were found to be normal. 



The nature of the substances inoculated into this control animal (6 c. c. of cipial parts of steril- 

 ized glycerine and distilled water) and the antiseptic precautions observed in the inoculation make 

 it doubtful whether the source of infection is traceable to the e.xjieriment. The changes noted in 

 the liver are of such a nature as to indicate their production by causes preceding even those which 

 brought about the death of the animal. 



Rabbit No. 2 of the first series, having received 2 c. c. of the fluid obtained by aspirating the 

 air of the hospital ward through the condensing apparatus moistened with sterilized glycerine, 

 died after 35 days. Autopsy : Half-grown rabbit, poorly nourished, and adipose all used up, 

 presented nothing important externally. Internally : A small amount of clear fluid in the abdomi- 

 nal cavity ; the liver is somewhat darker than normal, mottled, and contains a few psorosperms. 

 Spleen is normal. Kidneys and adrenals are normal in appearance. The right lung is considerably 

 congested, being readily torn ; the left is also slightly congested. The right side of the heart is 

 filled with dark fluid blood ; the left side is nearly empty. Several echinococcus cysts were found 

 in tjie abdominal cavity. 



Cover-slip preparations were made from the abdominal fluid, the kidneys, liver, spleen, lung, 

 and blood ; all proved negative. 



The organs were preserved in alcohol and mounted in celloidin for microscopic examination. 



Microscopic examination of the organs : Left lung showed the capillaries and larger vessels 

 very much dilated and filled with blood. Infiltration of leucocytes was noted here and there. 

 Right lung showed marked proliferation of cells and infiltration of leucocytes. Many of the air 

 cells were obliterated. The liver, kidneys, and spleen were normal. 



Rabbit No. i of the second series, inoculated with the fluid obtained from the air of the hospital 

 ward, died after 38 days. Auto])sy : Full-grown rabbit, shows numerous bruises and lacerations of 

 the skin over various parts of the body. Many of the wounds along the sides and back show 

 ecchymoses under the skin. Adipose not all used up. Internally : Liver slightly darker and some- 

 what larger, apparently, than normal. Spleen is larger than normal. Kidneys embedded in fat, 

 normal in appearance. Lungs and heart normal. Blood is dark and fluid. 



Cover-slips were made from all the organs vi-ith negative results. 



The organs were preserved in alcohol and mounted in celloidin for microscopic examination. 



Microscopic examination of the organs : No abnormalities could be found in any of the organs ; 

 all appearing to be normal. 



The remaining rabbits of these series showed no symptoms of any deleterious influence from 

 the fluid inoculated. No swelling or formation of abscess was noted in any of them. 



Rabbit No. 2 of the first series evidently died of lung disease, as shown at/^.f^ mortem. As to 

 the causation of this disease, it is impossible to venture an opinion. Rabbit No. i of the second 

 series died of causes which left apparently no manifestations pointing to their nature.* Rabbit No. 

 3 (control) of the third series evidently died from the effects of the extensive axillary abscess. As to 

 the source of the infection, no decided opinion can be given. Probably the infection gained an 

 entrance through the inoculation wound. 



Some experiments were made to determine the amount of oxidizable matter in atmospheric air. 

 At first a measured amount of air was slowly aspirated through twice distilled water, and the amount 

 of oxidizable matter extracted from the air estim.ated according to the method used for deterrain- 



*Death may have resulted from injury, as shown by the contusions and wounds noted at autopsy. These wounds 

 were probably inflicted by other rabbits in the same cage. 



