AND ITS EFFECTS UPON ANIMAL LIFK. 



53 



The heart invariably contained large, firm blood-clots, dark in color, extending from the auricles 

 into the ventricles. This was usually most marked on the right side. 



Microscopic examination of the organs, hardened in alcohol and mounted in celloidin, pre- 

 sented no other constant conditions than those brought about by the mode of death — the extensive 

 venous engorgement. The very slight pathological changes noted in isolated cases, from the 

 rapidity with which death ensued on exposure to the atmospheric conditions present, must be 

 attributed to causes antedating the time of the experiment by a considerable period. The changes 

 here referred to were mostly of the nature of interstitial changes present in the liver and kidneys. 

 No trace of the poisonous effects of any other respiratory products was noted in any of the animals 

 examined. 



The results obtained strengthened to a satisfactory degree the conclusions drawn from the 

 results obtained in the other experiments reported on. It was shown that in the absence of a suffi- 

 cient proportion of O in the artificial gaseous mixture to support life — at least 5 per cent. — the 

 animal speedily succumbed. On the other hand, CO, could be jjresent in quite large proportions, 

 as long as sufficient O was also present to su[)port life for some time, and no untoward effects were 

 manifested. The different animals used in these experiments — sparrows, rats, mice, guinea-pigs, 

 and rabbits — manifested no distinct differences in susceptibility to the conditions present. 



VI. — Experiments in the inoculation of animals with the moistune condensed from the 

 exhaled breath, as conducted by Brown-Sequard and d'Arsonval, by Hofmann-Wellenhoff, and 

 others. Four series of animals were inoculated with the fluid as shown in Table L. 



Series I. — The fluid, clear, limpid in character and without odor, of which 21 c. c. had been 

 collected from the breath of a healthy person on December 5, 1893, was warmed by holding the 

 receptacle containing it in a vessel of warm water, about 35" C. A rabbit, weighing 1870 g., 

 received if c. c. into the large vein at the margin of the ear. Another rabbit, weighing 1820 g., 

 also received if c c. in the same manner. A guinea-pig, weighing 220 g., received 4-h c. c. into 

 the peritoneal cavity. A second guinea-pig, weighing 280 g., also received 4^ c. c. into the peri- 

 toneal cavity. A third guinea-pig, weighing 220 g., received 4^^ c. c. of sterilized distilled water 

 into the peritoneal cavity as a control. 



These animals were kept under careful observation for more than a month, and as nothing 

 unusual in their condition presented itself, they were released. 



Series II. — On January 18, 1894, 20 c. c. of the fluid had been condensed from the breath of 

 the man having the tracheal fistula. The fluid was warmed by holding the receptacle containing it 

 in a vessel of warm water, about 36° C. 



Of this fluid 5 c. c. were injected into the peritoneal cavity of each of three white rats ; a fourth 

 rat receiving 5 c. c. of sterilized distilled water into the peritoneal cavity as a control experiment. 



inoculations with condensed fluid of expired breath. 



Table L. 



SERIES 1. 



Amount of fluid 

 injected. 



If c.c. 

 It 



4* 

 4i 

 4l 



Remarks. 



Under observation over a month. Healthy. 



Control — inoculated with sterilized distilled 

 water. 



