54 



xnK coNri'OsiTiox of expired air. 



SERIES II. 



5 t.L. 



5 " 



biill alive and healthy. 

 Died 9-6, 1894, from other causes. 

 iSiill alive and healthy. 



Control— inoculated with sterilized distilled 

 water. 



SEKIEs 111. 



Icb. 2 Rabbit 



1500 



2150 



880 



900 



7i c.c. jKilled after 48 days. 



10 " iStill under observation. Healthy. 



5 " JDied after 28 days. 



Control — inoculated with sterilized distilled 

 c " water. 



SERIES IV. 



iMch. 30 



Rabbit 



Still under observation. Healthy. 

 Killed 11-2, 1894. Healthy. 



Istill under observation. Healthy. 



These animals were under close observation for several months without noting any alteration 

 in their condition. One of them has since died (Sept. 6, 1894) from other causes. The others 

 continue well. 



Series III. — On February i, 1894, 44 r.c. of the fluid had been collected from the^ exhalations 

 of the man having the tracheal fistula. This fluid was again warmed, as before, to about 35° C, 

 and injected into the peritoneal cavity of rabbits as follows : 



No. 1. \\'eight, 1500 g., 75 c.c. of fluid. 



No. 2. " 2150 g., 1 0.0 c.c. " 



No. 3. " 880 g., 5 o c.c. " " 



No. 4. " 900 g., 5.0 c.c. " sterilized distilled water. 



Rabbit No. 3 of this series died during the night of March 4, 1S94, and an autopsy held the 

 ne.xt morning showed the following conditions* : 



Young female rabbit. Externally: Not very thin, adipose not quite used up. Internally: 

 On opening the abdominal cavity the organs were found in normal position. Stomach and large 

 intestines well filled. Liver slightly enlarged, no spots ; shows lobular apjiearance well marked ; 

 rather pale in color, as are all the organs and tissues (albino). (lall bladder well filled with pale 

 bile. Small intestines moderately filled ; no change in their ap|)earance ; Teyer's patches not 

 enl irged. Appendix not inflamed. Spleen not enlarged. Kidneys normal in size Adrenals small. 

 Lungs normal, rather pale. Heart rather pale, contracted on left side, right side filled with blood. 



Cultures were taken from the liver, spleen, blood, and abdominal fluid and all proved negative. 



Microscopic examination of the organs : Kidney : Presents some blood-vessels which contain 

 an increased amount of white blood corpuscles. C.lomeruli are slightly swollen, showing a small 



♦ Autopsy made by Dr. Olmsted. 



