AND ITS EFFECTS UPON ANIMAL LIFE. 55 



amount of infiltration. Slight increase of connective tissue between the tubules. Large blood- 

 vessels are very much dilated. Areas of slight e.xtravasation. A certain amount of cloudy swell- 

 ing. Liver — Shows large number of small areas of cell-death — necrotic areas. Breaking up of 

 cells and fragmentation of the nuclei, which is almost identical with the conditions found in 

 diphtheria. Adrenals — No change apjjarent. Sjileen — No change apparent. The teased heart 

 muscle, treated with acetic acid, shows possibly a trace of fatty degeneration. No "widespread 

 ecchymoses and hemorrhages in the lungs and intestines " were found, as reported by Brown-Sequard 

 and d'Arsonval. 



t)n March 20, i8()4, rabbit No. i of this series was killed in order to study the condition of its 

 organs and comjiare the results with the conditions found in rabbit No. 3. Weight before death, 

 1830 g., gain 330 g. It seemed to be in ijcrfect health. 



On opening the abdominal cavity the organs were found in normal position. No increase of 

 peritoneal fluid. On the liver a number of points (psorosperms ?), one a depression I mm. in depth, 

 grayish-white in appearance, were noted ; mostly on the left lobe. Several other small areas — 

 whitish in appearance, sharply limited in their outline, smaller than the last, not distinctly depressed, 

 usually two, three, or more together — were found scattered over the upper and lower surfaces of 

 the liver. The liver is dark in color, lobules well marked out ; of about normal size and consistency. 

 Cutting into the liver there is the usual amount of hemorrhage. Spleen — Small, if anything, it is 

 contracted, otherwise of normal appearance. Adrenals appear normal. Kidneys — Embedded in 

 usual amount of fat Normal in size, color, and consistency. Small echinococcus cyst in the great 

 omentum, and another in the liver. Litestines normal in appearance. Heart normal in appearance. 

 Portion of muscle teased with salt solution and treated with acetic acid shows no fatty change. 

 Lungs normal in appearance. 



Cultures were taken from the peritoneal tluid, liver, spleen, kidneys, and blood. All proved 

 negative. 



Microscopic examination of the organs : Liver — Contains a small hemorrhage at the depressed 

 part noted at autopsy. The other spots noted are found to be entirely superficial. Slight increase 

 of connective-tissue elements. Engorgement of a capillary noted. Kidney — Nephritis manifested 

 by some congestion of vessels, proliferation of the connective-tissue cells between the tubules and 

 around the glomeruli ; an occasional glomerulus being quite contracted. Spleen shows an increased 

 amount of pigment. 



The remaining rabbits of this series have continued well to the present time. 



Series IV. — On March 30, 1S94, 45 c. c. of the condensed fluid had been collected from the 

 breath of a healthy person. This was again warmed to 35° C, and injected into the peritoneal 

 cavities of four rabbits, each receiving 10 c. c. of the fluid ; their weights were as follows : 1 161 g., 

 1359 g-> 1400 g., and 1759 g. 



On November 2, 1S94, the rabbits of this series having remained healthy, Nos. 2 and 3 were 

 killed in order to study the condition of their organs, and determine whether they presented or- 

 ganic lesions traceable to the fluid injected. They were in perfect health as far as might be judged 

 from their appearances. 



On post-mortem examination all the organs in these animals were found to be normal. Nor 

 was any abnormality to be noted in microscopic examination of the organs. 



The remaining animals of this series continue well to the present time. 



The pathological conditions noted in the cases of rabbits Nos. i and 3 of Series III., are not 

 unusual in these animals, as they are very commonly found in normal animals reared in the labora- 

 tory and in those purchased from dealers.* It is unsafe to infer, therefore, that any of the condi- 

 tions noted in these animals were due to the action of the fluid injected. 



The sterility of the fluid injected into the animals in this series of experiments was tested each 

 time by the inoculation of portions of it into tubes of melted gelatin ; these were then hardened 

 according to Esmarch's method. In two instances several colonies of a yellow bacillus, common to 



* This fact has also been noted by Dr. Abbott. His observations have not yet been published. 



