Journal of Agricultural Research 



\o\. IV, No. I 



Twelve young tomato plants, 30 to 40 cm. high, blossoming and with 

 fruit set, were selected for one experiment. The plants were growing 

 in pots in the greenhouse and were in a healthy condition. 



A fungous suspension was made by pouring 10 c. c. of sterilized water 

 into 53-day-old cotton-stem cultures of the fungus which were rich in 

 pycnidia and spores. The culture tubes were well shaken, after which 

 the suspension was poured off into clean tubes. 



Three methods of inoculation were used : 



(i) The spore suspension was transferred by means of a camel's-hair 

 brush to the fruit of two plants. Care was taken not to injure the tissue 

 of one plant while needle pricks were made into the fruit of the other. 



(2) By means of an atomizer the suspension was sprayed upon four 

 plants, thoroughly wetting the fruit. Two of these plants were not 

 wounded and two were injured after spraying by pricking the fruit with 

 a sterilized needle. 



(3) Direct needle-prick inoculations from cotton-stem cultures of the 

 fungus were made into the fruit of three plants. 



Three plants having fruit pricked with a sterilized needle were set aside 

 as controls. During the first 24 hours these 12 plants were protected 

 from sunlight by strips of thick manila paper. Observations were made 

 every few days and records taken at the end of 6, 12, 24, and 28 days. 

 The results are given in Table I. 



Table I. 



'Results of inoculations of young green tomato fruit' with Phoma fungus 

 {greenhouse ex l>eriment^ 



