Apr. IS, 1915 



Disinfection of Hides 



77 



The test preparations used in experiment 14 were made as follows: 

 Pieces of hide were cut so as to weigh about 2>^ gm. A good-sized drop 

 of guinea-pig blood was allowed to fall upon the center of each piece, and, 

 before this clotted, a loopful of a suspension of anthrax spores was 

 thoroughly mixed in. The suspension of spores was obtained by rubbing 

 up in sterile water enough of the surface growth of an agar culture of 

 Bacillus anthracis obtained directly from the spleen of a guinea pig 

 (No. 25386) to give a suspension rather more dense than a 24-hour 

 bouillon culture of B. typhosus. The loop employed was of No. 23 gauge 

 platinum wire 3 mm. in diameter. The pieces of hide thus infected were 

 dried in an electric oven at a temperature of about 45° C, in order to 

 prevent the spores from developing into vegetative forms, which would 

 be destroyed by the drying. 



In experiment 15 (Table VIII) the test pieces of hide were prepared as 

 follows: Pieces cut to weigh 2^ gm. were placed in a rather dense sus- 

 pension of anthrax spores with hair side down. After soaking in this 

 solution for 10 minutes they were placed in Petri dishes hair side up and 

 allowed to dry a few minutes. Then o.i c. c. of the spore suspension 

 was dropped on each piece and they were allowed to stand at room tem- 

 perature for one hour. The pieces of hide were then dried in an electric 

 oven at 43° C. for two days, the covers of the Petri dishes being tilted 

 to one side. They were then kept at room temperature until used. 

 After exposure to the disinfectant a considerable part of the hair with 

 some of the underlying hide was scraped ofif and inoculated subcuta- 

 neously into guinea pigs, instead of inoculating blood clots as before. 

 In other respects the technique was the same as for experiment 14. 



Table VIII. — Inoculation of guinea pigs with poriiont of hide 



EXPERIMENT 15 



25717 

 25718 

 25719 

 25720 

 25721 

 25722 

 25723 



25724 



EXPERIMENT 16 



Mercuric chlorid (i:soo)+fonnic acid (i per cent) 



Do 



Mercuric chlorid (i:i,ooo)-|-formic acid (i per cent) 



Do 



Mercuric chlorid (i:2,ooo)-Momiic acid (i per cent) 



Do .•-. 



Sodium chlorid followed by sodium sulphid. No dis- 

 infectant. 



Do 



a Not exposed. 



(°) 



C) 



Lived. 



Do. 



Do. 



Do. 



Do. 



Do. 

 Died after 4 days 



Do. 



Anthrax. 



