78 



Journal of Agricultural Research 



Vol. IV, No. I 



Table VIII. — Inoculation of guinea pigs with portions of hide — Continued 



EXPERIMENT 17 



EXPERIMENT 18 



27221 

 27222 



27223 

 27224 

 27225 

 27226 

 27227 

 27228 

 27231 



27232 



Mercuric chlorid (i:2So)+formic add (i per cent). 

 Do 



Mercuric chlorid (i:soo)+fonmc acid (i per cent) 



Do 



Mercuric chlorid (1:1, ooo)+fomuc acid (i percent) 



Do 



Mercuric chlorid (1:2, 000)+ formic acid (i per cent) 



Do 



Sodium chlorid followed by sodium sulphid. No dis- 

 infectant. 



Do 



C) 

 (°) 



Died after 4 days. Anthrax. 

 Died after 6 days. Not 



anthrax. 



Died after s days. Anthrax. 



Died after 6 days. Anthrax. 



Died after 5 days. Anthrax. 

 Lived. 



Died after s days. Anthrax. 



Died after 6 days. Anthrax. 



Died after s days. Anthrax. 



Died. Mixed infection. 



"Not exposed. 



Experiment i6 (Table VIII) was similar to the preceding, except that 

 the pieces of hide used were dried for three instead of two days. A cul- 

 ture from the heart blood of guinea pig 25515 was used in making the 

 spore suspension. 



Apparently the added duration of drying had an injurious action upon 

 the spores. It should be noted, however, that cultures from one guinea 

 pig (No. 25386) were used in preparing material in experiments 14 and 

 15, while the test preparations used in experiment 16 were infected by a 

 culture derived from a dififerent animal. 



The available cultures from the same source as those used in preparing 

 material for experiments 14 and 15 were now 1 month old. In experi- 

 ment 17 (Table VIII) ope of these was used in infecting pieces of hide in 

 the following way: Pieces of hide of 2>^ gm. weight were soaked in a 

 suspension of anthrax spores for 10 minutes; then one-tenth c. c. of 

 suspension was dropped on each, and the pieces of hide were dried in an 

 electric oven at 43° C. for 24 hours and then kept at room temperature 

 for 24 hours before use. The covers of the Petri dishes containing the 

 pieces of hide were kept raised during all of this time. 



The results of this experiment seem to indicate that cultures derived 

 from one animal (guinea pig 25386) yielded spores of very great resisting 

 power as compared with cultures from another animal (guinea pig 25515). 

 The irregularities which will be noted in experiment 17 are probably due 

 to the age of the culture used. 



