So 



Journal of Agricultural Research 



Vol. IV, No. I 



In this experiment, as in those of similar character preceding it, neu- 

 tralization of the disinfectant by sodium sulphid was done within a com- 

 paratively few hours after the process of disinfection was complete. In 

 view of the strong dilution (i to 250) found to be inefficient under these 

 circumstances, no further attempt was made to find a dilution strong 

 enough to disinfect, with neutralization afterward. Instead of this, an 

 attempt was now made to determine how long spores remained ^dable 

 after treatment of the pieces of hide by much weaker dilutions of mer- 

 curic chlorid plus formic acid. This seemed worth while because the 

 Seymour-Jones method was originally proposed to be employed at for- 

 eign ports, and in a voyage of ordinary length a considerable time would 

 thus elapse between the time of disinfection and time of arrival at desti- 

 nation. 



In experiment 19 (Table IX) a number of pieces of hide were exposed 

 for 24 hours to mercuric chlorid, i to 4,000, plus i per cent of formic acid, 

 treated with saturated common salt for i hour, and then laid aside and at 

 intervals treated with sodium sulphid and inoculated into guinea pigs. 

 In each case they were treated with i per cent of sodium sulphid for 2 

 hours and washed with sterile distilled water. Material was then scraped 

 from each piece and inoculated subcutaneously into a guinea pig. 



Table IX. — Inoculation of guinea pigs with infected portions of hide 



EXPERIMENT 19 1 



EXPERIMENT 20 6 



27521 



27522 



27525 

 27526 

 27531 

 27532 



28004 

 28005 



Mercuric chlorid (i:4,ooo)+formic acid (i 

 per cent). 



Do 



Do 



Do 



Do 



Do 



Do 



Do 



Lived. 



Do. 



Died after 6 days. Anthrax. 

 Lived. 



Died after 4 days. Anthrax. 



Died after 5 days. Anthrax . 



Lived. 

 Do. 



1 Control guinea pig died of anthrax in 5 days. 

 b Control guinea pig died of anthrax in 7 days. 



