Apr. IS, 191S 



Disinfection of Hides 



81 



Table IX. — Inoculation of guinea pigs with infected portions of hide — Continued 



EXPERIMENT 21 1 



EXPERIMENT 22 6 



28013 

 28008 



28009 

 28052 

 28053 

 28054 

 280SS 

 28058 

 28059 



Mercuric chlorid (i:4,ooo)+formic acid (i 

 per cent). 



Do 



Mercuric chlorid (1:2, 500)-!- formic acid (i 

 per cent). 



Do 



Do 



Do 



Do 



Do 



Do 



Do 



Died after 9 days. Anthrax. 



Lived. 



Died after 13 days. Anthrax. 



Lived. 



Do. 



Do. 

 Died after 6 days. Anthrax. 

 Lived. » 



Do. 



Do. 



o Control guinea pig died of anthrax in 4 days. 

 6 Control guinea pig died of mixed infection. 



The irregular results noted above might be due to variation in the 

 extent of infection of the various pieces of hide. 



In another experiment with similar technique, except that the pieces 

 of hide were infected at a different time and by a different culture, the 

 results were as given in Table IX, experiment 20. 



In experiment 21 (Table IX), also, the procedure was the same as in 

 experiment 19, except that the test pieces of hide were infected by a 

 different culture. 



Experiment 22 (Table IX) was similar to the preceding experiments, 

 except in the use of a stronger dilution of the disinfectant. 



In connection with experiments 20, 21, and 22 part of the material 

 scraped from the pieces of hide was plated out to determine whether 

 sterilization had been accomplished. Growth of some kind was obtained 

 in every instance, although Bacillus anthracis was isolated in only about 

 one-third of the cases. In one instance B. anthracis was recovered from 

 material which failed to cause anthrax when inoculated into guinea pigs, 

 but on the other hand, one guinea pig died from anthrax after inocula- 

 tion with material which failed to yield B. anthracis by the plate method. 



