84 



Journal of Agricultural Research 



Vol. IV, No. I 



Table X.^ — Germicidal efficiency of hydrochloric acid plies sodium chlorid and mercuric 

 chlorid, with and without formic acid, and with acetic acid, by the rod method, without 

 addition of organic matter — Continued 



EXPERIMENT 25 — continued 



Rod 

 No. 



Disinfectant (10 c. c.) and dilution. 



Hydrochloric acid (5 per cent)+sodium chlorid (20 per cent) 



16 Mercuric chlorid (1:500) alone 



17 Mercuric chlorid (1:1,000) alone 



18 Mercuric chlorid (1:1, 000) + acetic acid (i per cent) 



19 Mercuric chlorid (i:2,ooo)+acetic acid (i percent) 



Mercuric chlorid (i:3,ooo)+acetic acid (i per cent) 



Mercuric chlorid (i:i,ooo)+formic acid (i per cent) 



Mercuric chlorid (i:2,ooo)+formic acid (i per cent) 



Mercuric chlorid (i:3,ooo)+fonnic acid (i per cent) 



Control rod 



" Not exposed. 



A similar experiment (Table X, experiment 25) was made with rods 

 infected by a spore suspension of about the same density as a 24-hour 

 bouillon culture of Bacillus typhosus. 



In experiments 26 and 27 (Table XI) are shown a comparison of hydro- 

 chloric acid and common salt with several other disinfectants, all with 

 24-hour exposure. Three rods were used with each dilution, showing 

 the result, respectively, when no defibrinated blood was added, with 

 ^2 c. c. of blood added to each tube and with i c. c. of blood added to each 

 tube. The hydrochloric-a<Jid rods were washed with a 2 per cent sodium- 

 carbonate solution, the mercuric -chlorid rods with a saturated hydrogen- 

 sulphid solution, and the formalin and carbolic-acid rods with distilled 

 water. 



Table XI. — Germicidal efficiency of hydrochloric acid plus sodium chlorid, fortnalin, 

 phenol, and mercuric chlorid, with and without formic acid, by Hie rod method 



EXPERIMENT 26 



Disinfectant (lo c. c.) and dilution. 



Time of 

 exposure. 



Result. 



No blood 

 added. 



H c. c. blood 

 added. 



I c. c. blood 

 added. 



Hydrochloric acid (i per cent)+sodiuin chlorid 



(10 per cent). 

 Hydrochloric acid (2 per c€nt)+sodiuin chlorid 



(10 per cent). 

 Hydrochloric acid (3 per cent)+sodiuin chlorid 



(lo per cent). 

 Mercuric chlorid (i:i,ooo)+fonnic acid (i per 



cent). 

 Mercuric chlorid (i:2,ooo)+forTnic acid (i per 



cent). 

 Mercuric chlorid (i:4,ooo)+formic acid (i per 



cent). 

 Mercuric chlorid (i:8,ooo)+fonnic acid (i per 



cent). 

 Merctuic chlorid (i:i6,ooo)+formic acid (i per 



cent). 



Formalin (1:50) 



Formalin (1:100) 



Formalin (1:230) 



Formalin (i;i,ooo) 



Phenol (5 per cent) 



Hours. 



No growth . 



do 



do 



do 



do 



do 



do 



do 



No growth. 



do 



....do 



do 



do 



Growth . . . . 



do 



do 



do. . 



do. . 



Growth . 



do. . 



do. . 



No growth . . 



Growth 



do 



do 



do 



No growth. 



Do. 



Do. 

 Growth. 



Do. 



Do. 



Do. 



Do. 



Do. 

 Do. 

 Do. 

 Do. 

 Do. 



