Apr. IS, 1915 



Disinfection of Hides 



85 



Table XI. — Germicidal efficiency of hydrochloric acid plus sodium chlorid, formalin, 

 phenol, and mercuric chlorid, -with and without formic acid, by rod method — Continued 



EXPERIMENT 27 



Disinfectant (10 c. c.) and dilution. 



Hydrochloric acid (i per cent)+sodium chlorid (10 per cent). . . 

 Hydrochloric acid (2 per cent) + sodium chlorid (10 per cent) . . . 



Mercuric chlorid (i:i,ooo)+formic acid (i per cent) 



Mercuric chlorid (i;2,ooo)+formicacid (i percent) 



Mercuric chlorid (r:4,ooo)+fonnic acid {i per cent) 



Mercuric chlorid (i;6,ooo)+fonnic acid (i per cent) 



Mercuric chlorid (i:8,ooo)+formic acid (i per cent) 



Formalin (1:50) 



Formalin (1:100) 



Formalin (1:200) 



Time of 

 exposure. 



Hours. 



Result. 



14 c. c. blood 

 added. 



No growth . 



....do 



....do 



....do 



Growth . . . . 



....do 



...do 



No growth . 

 Growth . . . . 

 ....do 



I c. c. blood 

 added. 



No growth. 



Do. 



Do. 

 Growth. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



The technique of experiment 27 was the same as that of No. 26. In 

 this case two rods were used with each dilution, showing results with 

 )4 c. c. and i c. c. of defibrinated blood. 



II. EXPERIMENTS UPON PIECES OF HIDE INFECTED WITH SPORES OF BACILLUS 



ANTHRACIS 



In experiment 28 (Table XII) a 2 per cent hydrochloric-acid solution 

 plus 10 per cent of sodium chlorid was used with a 48-hour exposure, 

 25 c. c. of the disinfectant being used for each piece of hide. After 

 exposure the pieces of hide were soaked for 15 minutes in a 3 per cent 

 solution of sodium carbonate (25 c. c. for each). The pieces of hide used 

 were prepared by the method given by Ponder (9, 10) and were part of 

 the same lot as the pieces used in experiment 1 4. After disinfection the 

 clots were scraped off and inoculated subcutaneously into guinea pigs. 



Table XII. — Inoculation of guinea pigs with clots scraped from pieces of hide 



EXPERIMENT 28 



Guinea 

 pig No. 



Disinfectant (25 c. c.) and dilution. 



Result of inocu- 

 lation. 



25556 



25SS7 

 25558 

 25559 

 25560 

 25561 

 25562 

 25563 

 25554 

 25555 



Hydrochloric acid (2 per cent)+sodium chlorid (10 per 

 cent). 



Do 



Do 



Do 



Do 



Do 



Do 



Do 



No disinfectant 



Do 



Lived. 



Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Died. Anthrax. 

 Do. 



"Not exposed. 



