86 



Journal of Agricultural Research 



Vol. IV, No. I 



Experiment 29 (Table XIII) was made as follows: Pieces of hide were 

 prepared by soaking in spore suspension and then drying in an electric 

 oven. Details given in connection with experiment 15 will apply to 

 this experiment. The technique otherwise was the same as that of 

 experiment 28. Material w^as scraped from the surface of each piece and 

 inoculated subcutaneously into guinea pigs. 



Table XIII. — Inoculation of guinea pigs with material scraped from pieces of hide 



EXPERIMENT 29 



Guinea 

 pig No. 



25710 



25711 

 23712 

 25713 

 25714 

 25715 

 25716 



Disinfectant (25 c. c.) and dilution. 



Hydrochloric acid (2 per cent) + soditun chlorid (10 

 per cent). 



Do 



Do 



Do 



Do 



No disinfectant 



Do 



EXPERIMENT 30 



Hydrochloric acid (2 per cent) + sodium chlorid (10 

 per cent). 



Do 



Do 



Do 



Do 



Do 



Do 



Do 



Do 



Do , 



No disinfectant 



Do 



EXPERIMENT 31 



Time of 

 exposure. 



Hours. 



48 



(a) 

 C) 



Result of inoculation. 



Lived. 



Do. 



Do. 



Do. 



Do. 

 Died in 3K days. 

 Died in 6 days. 



Anthrax. 

 Anthrax. 



25733 



2S734 

 25735 

 25736 

 25737 

 25738 

 25739 

 25740 

 25741 

 24742 

 25729 

 25730 



48 Lived. 



48 



(«) 

 («) 



48 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 Died. Mixed infection. 

 Died after s days. Anthrax. 



n Not exposed. 



Experiment 30 (Table XIII) was made upon pieces of hide prepared 

 in the same way but infected with a different culture. 



In experiment 31 (Table XIII) the pieces of hide were prepared by 

 soaking in spore suspension and drying them over sulphuric acid in a 

 vacuum at 10° C. for 48 hours. As before, each piece of hide after dis- 

 infection was immersed for 15 minutes in 25 c. c. of a 3 per cent sodium- 

 carbonate solution. 



In connection with experiment 31 an attempt was made to determine 

 the efficiency of disinfection by plating out material from the piece of 



