462 



Journal of Agricultural Research 



Vol. IV, No. s 



beakers and precipitates washed a number of times with a 2J2 per cent ammonium- 

 hydroxid solution. The inside filters and precipitates were returned to their respec- 

 tive beakers, treated with 25 c. c. of dilute nitric acid, and the filters well shredded 

 with the stirring rods. Clean beakers were put under the funnels, the solutions 

 filtered through their respective funnels, and the filters washed repeatedly with 

 boiling distilled water. To each solution 15 c. c. of ammonia (sp. gr. 0.90) were added. 

 The solutions were then made slightly acid with pure nitric acid. The phosphorus 

 was precipitated with acid ammonium molybdate and the phosphorus determination 

 continued as usual. 



{e) Organic aciehsolublE phosphorus was determined by subtracting the in- 

 organic acid-soluble phosphorus from the total acid-soluble phosphorus. 



It should be said in this connection that since these different forms of 

 phosphorus were determined in connection with this investigation it has 

 been clearly demonstrated in this and other laboratories that the above 

 methods, or, in fact, any other methods at present known for the separa- 

 tion and estimation of inorganic and organic phosphorus in vegetable 

 substances, are not strictly accurate and give only approximate results. 

 It is the opinion of the authors that the above methods are as accurate 

 for this purpose as any known at present and that the results obtained 

 are probably a fair approximation to the true values for the inorganic 

 and organic phosphorus in the materials examined. 



LIVE WEIGHTS AND TOTAL GAINS IX BODY WEIGHT 



The lambs were weighed the three days immediately before they were 

 put into the digestion crates and the three days immediately following 

 the metabolism test. The average of each of these three weights is given 

 in Table I, together with the total gain in weight of each lamb from 

 December 15 to January 5. 



Table I. — Live -ueights and total gains in live ueigkts {in pounds) cf lambs in metab- 

 olism test 



WEIGHTS AND COMPOSITION OF FEEDS CONSUMED 



The quantities of the feeds consumed by the individual lambs during 

 the 12 days of the metabolism test, expressed in grams per day, are 

 given in Table II. Lamb 463 ingested a ration relatively very high in 

 roughage, while lambs 46S and 462 ingested a ration relatively low in 

 roughage. Lamb 458 ingested the largest quantity of total feed. The 

 chemical composition of the feeds consumed is given in Table III. 



