Sept. 15. igi; Soil Protozoa 551 



teenth day, and in the other solution they did not appear at all. For 

 the development of the greatest numbers of small ciliates at the differ- 

 ent temperatures, in the media inoculated with the various soils, the 

 greatest numbers developed in hay infusion at 29° to 30° C. with inocu- 

 lations of greenhouse and manured field soil, while with field soil the 

 maximum development was at 15° to 16° C. 



DEVELOPMENT OF LARGE CILIATES 



On examining Tables X, XI, and XII it at once becomes apparent 

 that either the soils examined contained very few large ciliate cysts or 

 the conditions in the media were unfavorable for their existence, as 

 very few of these organisms developed. Blood extract seemed a little 

 more favorable than hay infusion. In all the inoculations with the 

 greenhouse soil a lew large ciliates developed. In solutions of dried- 

 blood extract with the greenhouse soil at 6° to 7° they first appeared 

 on the fourteenth day, at 15° to 16° on the sixth, at 22° to 23° on the 

 fourth, and at 29° to 30° C. they did not appear until the ninth day 

 after inoculation. At 22° to 23° and 29° to 30° C. a few large ciliates 

 developed in the hay -infusion inoculations of greenhouse soil. With 

 the exception of a few organisms that appeared in the blood-extract 

 inoculations of the manured field soil at 15^ to 16° and in the hay- 

 infusion inoculations of field soil at 29° to 30° C. and in the one men- 

 tioned above, no large ciliates appeared in any of the solutions. 



DEVELOPMEXT OF FLAGELL.\TES 



Again the temperature plays a very important role in the develop- 

 ment of flagellates. Upon considering the largest numbers of organ- 

 isms developed at different temperatures in dried-blood extract, it is 

 seen that in all cases the greatest development appeared at 6° to 7° 

 and the smallest at 29° to 30° C. There were 150 to 250 times as many 

 developed at the former temperature as at the latter. In comparing 

 all inoculations of hay infusion it is noted that the greatest develop- 

 ment for all the soils examined was at 15° to 16^; the smallest in some 

 cases occurred at 6° to 7°; in others at 29° to 30°. With one excep- 

 tion, at 6^ to 7°, greater numbers were developed in hay infusion than 

 in dried-blood extract. Comparing the greatest numbers of flagellates 

 developed at the different temperatures and in the various media, it is 

 seen that the greatest development for all soils was in hay infusion at 

 15° to 16° C. In all cases in the inoculations of hay infusion and in 

 most cases \\nth dried-blood extract at 6° to 7° these organisms did not 

 appear until the fifth day and the maximum was not reached until the 

 twentv-eighth or twentv-ninth dav after inoculation. 



