31 « The Carhohjidrnti'S of the Manyold Leaf 



(b) Inverlasc. 



50 cc. of C {= 15 cc. A) gave 0-3706 grm. CuO. 



Solution C" gave rotation = - 0-071° in 200 luni. tube at 20°. 



Mnllose. 



.'500 cc. of Solution A deprived of lead by hydrogen sulphide were 

 diluted to 500 cc. = Solution B'. 



Three 50 cc. portions of Solution B' were fermented by S. cxiguus, 

 S. marxianus and .S'. mwmalus. After treatment with 5 cc. of 

 alumina cream each solution was made to 100 cc. ; 50 cc. of the filtrate 

 (= 15 cc. A) gave 0-0134 gnu. CuO (average of the three). 



Two 50 cc. portions were fermented similarly with distillery yeast. 

 50 cc. of the final solution (= 15 cc. A) gave 0-01:^7 giiii. CuO (average 

 of the two). 



Pentoses. 



50 cc. A gave 0-0149 grm. phloroglucide. 



Pentose = (0-0149 + 0-0052) x 1-017 x '^ x ^^ x ,,^?^„ = 0-68 % 



50 440 1.34-79 



on the T.v.D.M. 



Calculation of Results. 



Saccharose, (a) Citric acid. 



From reduction. Increase of reduction in 25 cc. B (= 15 cc. A) 

 caused by inversion 



= 0-3710 - 0-2210 = 0-1.500 giin. CuO. 



This corresponds, using the factor for 0-3710 grm. of CuO in Brown, 



Morris and Millar's Tables, to -^^n gi'ms. of invert sugar. 



% of saccharose on T.v■.l).^r. of leaf 



0-1500 ,,^, 2000 500 100 „ .. ., 

 = Tm " ''■^■' ^^ TF ^ 440 ^ 134T9 = ^-8° %• 



(b) Invertase. increase of reduction on inversion of 15 cc. A 



= 0-370(; 0-2210 0-1 lltdgrni. CuO. 



/. % of saccharose (as in (a)) -^ 6-78 %. 



