CERTAIN GENERA OF THE CLOSTRIDIACEAE 27 



delicacy. It maj' be that they are descended from organisms 

 of the genus Martellillus. 



Type species R. fragilis nov. sp. Characters of genus. 



I regret that the strain kindly sent me by Dr. Records died 

 soon after its arrival. As it had sporulated in meat medium 

 this was surprising, but was consistent with Dr. Records' de- 

 scription of the behavior of the organism. The strain was 

 isolated from a liver infarct in a cow. 



Genus 22. Tissierillus nov. gen. 



Putrificoideae that attack sugars and clot milk. Slender 

 Gram-negative or Gram-positive rods with oval terminal spores. 

 Colonies in deep agar have radiate periphery and opaque cen- 

 ter. Frequently intestinal saprophytes. 



Type species T. paraputrificus (Bacillus paraputrificns de- 

 fined by Bienstock) as described by Mcintosh under the name 

 Bacillus putrificus. Ferments glucose, maltose, lactose, su- 

 crose and starch. 



References to organisms of this type are made by Passini, 

 Moro, Metchuikoff and Kligler. Very likely some descriptions 

 of this type were based on the behaidor of mixed cultures. 



Genus 23. Putrificus nov. gen. 



Putrificoideae that do not attack sugars. Slender Gram-nega- 

 tive or Gram-positive rods with oval terminal spores. Colonies 

 in deep agar have radiate periphery and opaque center. Putre- 

 factive organisms found in soil and wounds. 



Type species P. Bienstocki (Bacillus putrificus Bienstock) 

 as defined by Bienstock (1906). References: Bienstock (1884, 

 1901, 1906), Klein, Rodella (1905). The latter differentiated 

 three types of what he termed B. putrificus. Tizzoni, Catani 

 and Baquis described two organisms of this general type. B. 

 poslumus of Wiircker is of this group. His B. putrificus is of 

 sporogenes affinities. I possess a strain (lOR) isolated by Miss 

 Robertson or myself from a wound, which is mildly proteolytic, 

 does not split sugars, is Gram-negative, and on serum produces 

 oval terminal spores. Its deep colonies are transparent, len- 



