STUDIES UPON AGGLUTINATION 47 



Bad. paratyphosum B. Formalinized Bnct. lyphosum senim 

 (1:200), practically did not show the pseudo agglutination; 

 most cases were negative (with the exception of no. 1 strain, 

 which had strong agglutination with this serum, and weak agglu- 

 tination with homologous serum, — a peculiar strain but never- 

 theless typical Bad. paralypJiosuDi B), while showing strong 

 agglutination without formalin. 



Bad. paratyphosum A senim (1:50); pseudo agglutination is 

 shown to be negative with formalin, but strong agglutination 

 occurs without formalin; hence, this serum does not have a 

 tendency to pseudo agglutination with Bad. paratyphosum B, 

 if used with formalin. 



Polyvalent dysentery serum (1: 100), or normal horse serum 

 (1 :50), shows negative or weak pseudo agglutination with forma- 

 lin, and strong agglutination without it. 



Bad. dysenteriae Shiga senmi; many strains are negative or 

 show a weak reaction with formalin, but show more pseudo 

 agglutination without formalin. This, however, is a strongly 

 concentrated sei-um (1 : 50), if it is diluted 1 : 100, it does not agglu- 

 tinate with Bad. paratyphosum B. 



Bad. coli. As a loile Bad typhosum serum (from immune rabbit 

 or sheep), with or without formalin, shows no agglutination in 

 high concentrations, as 1:200. 



Bad. paratyphosum A and B serum (from immune rabbit), 

 generally showed no agglutination with Bad. coli. 



Polyvalent dysentery serum showed strong agglutination 

 with Bad. coli either with or without formalin, almost equal to 

 that of the Bad. dysenteriae group, as observed by Kligler. This 

 serum we could use for a diagnostic test for Bad. coli. 



Bact dysenteriae Shiga serum (1:50), showed slight or negative 

 agglutination with formalin, but more without formalin; it is 

 however not a marked reaction. 



Bad. dysenteriae group. With Bad. typhosum serum (1:200), 

 and Bad.paratyphosumB serum (1:50), with or without formalin, 

 pseudo agglutination was generally negative, but with Bad. 

 paratyphosum A serum 1:50 (obtained from rabbit), there was 

 slight agglutination. 



