STUDIES UPON AGGLUTINATION 



61 



BacL typhosum, Bad. paratyphosum A and B, and the Bad. 

 coll group. 



In the case of Bad. typhosum and Bad. paratyphosum A and B, 

 spontaneous apghitinalion generally appears weak macroscop- 

 ically and strong microscopically; but with Bad. coli it appears 

 to be almost the same with either method. It is specially to 

 be noted that when using spontaneous agglutinating bacilli for 

 the Widal test, the macroscopic method was found more reliable. 



For the fundamental or graphic agglutination test, I used non- 

 spontaneous agglutinating bacilli. ^Tien agglutination was 



TABLE n 



Agglutination tests at different lemperatvres 



Key: 5, complete ; 4, almost complete ; 3, weak ; 2, very weak ; 1, trace ; 0, nega- 

 tive agglutination. 



weak or the case questionable, I resorted to the microscopic 

 method for final determination. 



AGGLUTINATION AT DIFFERENT TEMPERATURES (TABLE 11) 



Delepine, Jordan, Konrich, Lion, Meyer and Kilgore, Widal, 

 and many others conducted agglutination tests at 37°C. ; Dreyer 

 and Blake, Hetsch, Kolle, Kutscher, Forges, and Weil advise 

 the use of 50°C. to 55°C. ; Joos 35°C. to 40°C. ; BerUner and Cohn, 

 and Durham recommend room temperature. 



Recently many workers have read the test, after keeping the 

 tubes in the water bath two to three hours at from oO°C. to 55°C. 

 then leaving them at room temperature or in the cold room over 

 night. 



