78 



o. isHn 



2 strains (nos. 19 and 21), after frequent sub-culture in broth, 

 pepton water and agar throughout a period of one year, gave 1 

 type of spontaneously agghitinating bacilli during the entire 

 period. When, however, 1 per cent glucose broth was used for 

 daily transplants, culture no. 19 showed 2 types of bacilU after 

 seven days and culture no. 21 after eighteen days. 



A new strain of Bad. typhosum was isolated from the blood of 

 a patient at the Peter Bent Brigham Hospital in the spring of 



TABLE 3 

 Isolation of colonies from stock stab cultures of agar medium 



First isolation' 



Isolation of 

 subcultures 

 after several 

 months 



OaOANISM 



Bad. typhosum... 

 Bad. paraty- 



phosum A 



Bad. paraly- 



phosum B 



Bad. coll 



Bad. enteritidis . 

 Bad. dysenteriae 



Bad. typhosum.. 

 Bad. paraty- 



phosiim A 



Bad. paraty- 



phosum B 



Bad. coli 



Bad. enteritidis 

 Bad. dysenteriae 



DOUBTFDT. 

 AGGLUTI- 

 NATION 



1918. The cultures on agar plates gave pure smooth colonies 

 which when transplanted to broth showed non-spontaneously 

 agglutinated bacilli. A single colony was fished from an agar 

 plate and transplanted successively for a period of twelve days 

 in broth. At the end of the twelfth day it showed .the presence 

 of bacilli of the spontaneously and non-spontaneously aggluti- 

 nating types. 



Most of the colonies which developed after planting cultures 

 freshly obtained from animals showed non-spontaneous-agglu- 



