82 o. isHii 



bacilli daily exceeded that of spontaneously agglutinating bacilli; 

 others showed a more vigorous growth of the latter type, and 

 a few remained constant, showing parallel growth of the two 

 types. 



Other cultures which were not transplanted, showed a very 

 slow change, while the daily transplanted culture showed in 

 comparison very rapid changes in the proportion of the colony 

 types. This varied directly with the original strains of the 

 bacilli. 



SPECIFIC AGGLUTINATING POWER BETWEEN TWO TYPES OP 

 COLONIES OF ONE STRAIN 



In experiments on specific agglutination with different types 

 of colonies, no difference was found in the power of agglutination 

 with two types of colonies of one strain. 



Spontaneous agglutination differs chemically from specific 

 serum agglutination, in that spontaneous agglutination can be 

 prevented with most strains of Bad. typhosum and Bad. para- 

 typhosum A and to a lesser extent with Bad. paratyphosum 

 B, by adding 0.05 to 1 per cent formalin. Formalin in 0.05 to 

 0.2 per cent, does not interfere with specific serum agglutination, 

 but slightly increases it if plain salt solution (0.85 per cent) has 

 been used. 



Many spontaneously agglutinating strains of Bad. typhosum 

 when grown in glucose broth fail to show spontaneous agglutina- 

 tion. In others spontaneous agglutination is not inhibited by 

 this means; neither does growth in glucose broth destroy the 

 power of specific agglutination. 



NOTES ON AGGLUTINATION TEST 



In the agglutination test as applied to members of the colon- 

 typhoid group it is necessary to differentiate between organisms 

 giving spontaneous agglutination and organisms which do not 

 agglutinate spontaneously. Organisms of the first group ap- 

 pear to give specific serum agglutination, but in the control 

 bacillary suspension, when mixed with broth, pepton water, 



