INFLUENCE OF VACUUM UPON GROWTH OF BACTERIA 291 



TABLE 4 



Influence of varying amounts of acetic acid and air upon the growth of B. suhtilis in 



broth 



EXHAC8TBD 

 TO MM. Hg. 



50 



100 



per cent 



5 

 10 

 25 



5 

 10 

 25 



5 

 10 

 25 



Average . 



175 



350 



525 



685 



5 

 10 

 25 



5 

 10 

 25 



5 

 10 

 25 



5 

 10 

 25 



PER CENTS OP N/1 ACID AKD pH 



0.5 per cent pH 6.6 



Added 



715,000 

 783,000 

 716,000 



813,000 

 872, 000 

 783,000 



858, 000 

 813,000 

 783,000 



802,000 



Deter- 

 mined 



429,000 

 450,000 

 457,000 



392,000 

 465,000 

 472,000 



408,000 

 430,000 

 472,000 



800,000 

 S5S, 000 

 737,000 



907,000 



37,000 



813,000 



872, 000 

 858, 000 

 813, 000 



443.000 



409,000 

 383,000 

 409,000 



400,000 

 374,000 

 451, 000 



440,000 

 383,000 

 410,000 



463,000 

 400,000 

 391,000 



250, 000 

 250,000 

 303,000 



212,000 

 270,000 

 303,000 



266,400 

 259, 400 

 273,600 



276,000 

 258,000 



Average , 



409,400 



per cent pH 5.00 



Added 



173,000 



19,000 



265,000 



223,000 

 232,000 

 175,000 



209,000 

 250, 000 

 273,000 



836,000 



224,000 



410,000 

 151,000 

 383,000 



409,000 

 429,000 

 440,000 



366,000 

 429,000 

 409,000 



440,000 

 391,000 



216,000463,000 



262, 300 



Deter- 

 mined 



890, 000 



872,000 

 715,000 



800,000 

 828,000 

 761,000 



858,000 

 847,000 

 828,000 



214,000 

 270,000 

 190,000 



250, 000 

 230,000 

 137,000 



128, 000 

 178,000 

 210,000 



230,000 

 220, 000 

 225,000 



2 per cent pH 4.30 



Added I'^"".- 

 mined 



241,000 

 205,000 

 256,000 



234,000 

 247,000 



198,000 

 236, 000 

 234,000 



822,000 



410,000 

 409,000 

 451,000 



409,000 

 440,000 

 409,000 



409,000 

 463,000 

 366,000 



410,000 

 488,000 

 429,000 



418,000 



206,900424,400 



800,000 

 890,000 

 828,000 



&58, 000 

 858,000 

 800,000 



813,000 

 761,000 

 800,000 



231,000 



157,000 

 171,000 

 192,000 



148, 800 

 141,600 



162, 400 

 146, 400 

 165, 600 



176, 800 

 162, 400 

 158, 400 



4 per cent pH 4.00 



Added Srd 



823,000 



391, 000 

 391,000 

 400,000 



409,000 

 409,000 

 400,000 



451,000 

 391,000 

 409,000 



400,000 

 429,000 

 400,000 



162, 100 407, 700 



184,000 

 170,000 

 145,000 



176,000 

 166,000 

 186,000 



52,000 

 100,000 

 130,000 



146,000 



70,000 

 170,000 



140,000 

 103,000 

 210,000 



100,000 

 180,000 

 110,000 



140,000 

 190,000 

 131,000 



140,000 



upon the decrease in numbers, but the higher amounts of acid 

 were more active than the smaller amounts. As with B. suhtilis 

 in the presence of salt, there is a marked decrease even in the 

 tubes with smaller amounts, but unUke the higher amounts of 



