INFLUENCE OF VACUUM UPON GROWTH OF BACTERIA 295 



then cxliausted to the points desired and sealed at the mark. 

 The tubes were all placed in a steam sterilizer and heated for one 

 hour. Tables 8 and 9 show the results obtained. Since each 

 tube was of slightly different volume the liquid remaining after 

 removing the above amounts would contain slightly different 



TABLE 7 



Influence of vari/ing amounts of sail and air upon Ihe Ihermal death point of B. 

 mesenlericus spores. Original number of spores added 3,780,000 



numbers per cc. The reduction in numbers in this experiment 

 is so striking that the numbers are not included in the tables in 

 each case. The average number for each cc. of the liquid re- 

 maining in the tubes was about 37,400,000 per centimeter for 

 B. subtilis and 1,760,000 for B. mesentericus. 



