332 J. HOWAED MUELLER 



tated HgS was filtered off, and the hea\'ily pigmented filtrate 

 freed from Ba with H2SO4, and diluted to 100 cc. This crude 

 preparation was tested for activity as follows: 



(Decolorized infusion 25 cc. 

 Glucose-salt solution 25 cc. 

 HgSO« ppt. fraction 2.5 cc. ( = .25 gm. casein) 



Lot 2 Same plus HgSO« ppt. fraction.. .0.25 cc. ( = 0.025 gm. casein) 



J , o /Decolorized infusion 25 cc. 



[Glucose-salt solution 25 cc. 



To the remainder of the HgS04 precipitate fraction was added 

 10 cc. of a 10 per cent solution of HgS04, and after standing over 

 night, it was filtered. Both the precipitate and the filtrate were 

 freed from Hg and from H2SO4 as usual, and brought to a volume 

 of 100 cc. 



[Decolorized infusion 25 cc. 



_ . , I Glucose-salt solution 25 cc. 



Lot 1 < , 



plus 



[HgSO^filtrate fraction 1 .0 cc. 



Lot 2 Same plus filtrate fraction .5 cc. 



Lot 3 Same plus precipitate fraction 1 .0 cc. 



Lot 4 Same plus precipitate fraction .5 cc. 



Lot 5 Same plus each fraction 1 .0 cc. 



Lot 6 Same plus each fraction 0.5 cc. 



J . „ fDecolorized infusion 25 cc. 



\Glucose-salt solution 25 cc. 



