378 



L. D. BUSHNELL 



All the cultures were completely non-toxic when grown on 

 brain and liver broth and on brain — saline for different lengths 

 of time and injected subcutaneoush^ into guinea-pigs in 1 cc. 

 amounts. Table 2 shows the results of one series. 



TABLE 2 

 Residts of animal inoculation 



TABLE 3 



Agglutination reaction 



* Figures show amount of agglutination. 10 = complete. 



Several of the cultures of this organism were also serologically 

 similar to known cultures of B. sporogenes, as showTi by table 3. 

 Their non-toxic nature and the appearance of colonies in deep 

 agar would differentiate them from B. hotulinus, to which species 

 they are otherwise very smiilar. 



