402 L. D. BUSHNELL 



probably utilize a certain amount of free oxygen when it is 

 available. 



4. The quantitative results obtained in this particular case do 

 not happen to yield results of significance in the subdivision of the 

 group of organisms studied. They do, however, give us a clear 

 picture of the biochemical behavior of these types which will 

 make possible an accurate comparison of their physiology with 

 that of other forms which maj^ later be investigated. 



5. It may be hoped that chemists will interest them.selves in 

 the improvement and simplification of chemical methods neces- 

 sary to determine quantitatively and biochemically changes due 

 to the growth of microorganisms in various culture media. As 

 such methods are devised the bacteriologist will obtain an in- 

 creasingly sound and logical basis for the differentiation of micro- 

 bial species, and will obtain much valuable information concern- 

 ing the nature of the life processes of microorganisms. 



The author is indebted to Professor P. L. Gainey and Professor 

 0. W. Hunter of this laboratory for assistance in this work. 



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