418 



G. S. "mLSON 



one hours on each of them. The results have been plotted in the 

 form of a graph to render them more striking (fig. 1). The figures 

 are given in terms of the original culture. From this it will be 

 seen that while all three fluids have a deleterious effect on the 

 organisms, that of Ringer's solution is the least marked, and 

 saline the most pronounced, that of tap water occupying an 

 intermediate position. 



A fresh experiment was performed with the idea of discovering 

 what proportion of Ringer's solution was necessary to prevent 



COUNT IN 

 riiLkioNS 



200 



ioo 400 too aoo io<c lioo i<voo 



T I ME IN MINUTES. 



Fig. 1. Showing the Effect of VAnious Diluents on the Viability 



OP Bacteria 



the deleterious effect of distilled water on organisms emulsified 

 in it. A quantity'' of Ringer's solution was prepared according to 

 the following formula: 



Sodium chloride 0.9 grams 



Potassium chloride .042 gram 



Calcium chloride .048 grams 



Sodium bicarbonate .02 gram 



Distilled water 100.0 cc. 



From it five separate dilutions were made with distilled water, 

 commencing with one-half and passing by geometrical progres- 



