434 



G. S. WILSON 



to grow for more than three hours at 37°C., so as to maintain the 

 organisms in a medium of comparatively fresh broth. This again 

 was without effect. The viable count still persisted below the 

 total. No method, in fact, could be ascertained which would 

 abolish this inferiority, and it was thought that possibly the tech- 



EXPT tZ. 



300 400 



N n I NUTE5. 



(OS 



TIME 



Fig. 5 Showing the Total and Viable Counts in a Broth Culture of 



Bact. 6UIPESTIFER 



Total count = continuous line. Viable count = interrupted line 



nique or the caUbration of the instruments employed might be 

 at fault. But this idea had Ukewise to be given up when quite 

 unexpectedly the result of one experiment showed that during 

 at least fifty minutes of growth the two counts were identical. 

 The figures for this experiment are quoted in table 8 and figure 4. 

 The low total count at four hundred and twenty minutes was 

 probably due to a technical error. Apart from this it will be seen 



