VIABLE BACTERIA IN YOUNG CULTURES 445 



this time has been found to be shorter than that usually given for 

 cultures of similar organisms. 



It is with pleasure that I record my thanks firstly to Dr. W. \Y. 

 C. Toplcy for his constant help and advice throughout the prog- 

 ress of this work, and secondly to JMr. J. E. Barnard, of the 

 National Institute of Medical Research, for his assistance in the 

 calibration of the Holbe counting chamber employed in these 

 investigations. 



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